4zes: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Blood dendritic cell antigen 2 (BDCA-2) complexed with methyl-mannoside== | ==Blood dendritic cell antigen 2 (BDCA-2) complexed with methyl-mannoside== | ||
<StructureSection load='4zes' size='340' side='right' caption='[[4zes]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='4zes' size='340' side='right'caption='[[4zes]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4zes]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZES OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4zes]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZES FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zes OCA], [https://pdbe.org/4zes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zes RCSB], [https://www.ebi.ac.uk/pdbsum/4zes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zes ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/CLC4C_HUMAN CLC4C_HUMAN] Involved in antigen-capturing. Target ligand into antigen processing and peptide-loading compartments for presentation to T-cells. May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium. Does not seem to bind mannose.<ref>PMID:11748283</ref> <ref>PMID:11031109</ref> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Blood dendritic cell antigen 2 (BDCA-2; also designated CLEC4C or CD303) is uniquely expressed on plasmacytoid dendritic cells. Stimulation of BDCA-2 with antibodies leads to an anti-inflammatory response in these cells, but the natural ligands for the receptor are not known. The C-type carbohydrate-recognition domain (CRD) in the extracellular portion of BDCA-2 contains a signature motif typical of C-type animal lectins that bind mannose, glucose or GlcNAc, yet it has been reported that BDCA-2 binds selectively to galactose-terminated, bi-antennary N-linked glycans. A combination of glycan array analysis and binding competition studies with monosaccharides and natural and synthetic oligosaccharides have been used to define the binding epitope for BDCA-2 as the trisaccharides Galbeta1-3/4GlcNAcbeta1-2Man. X-ray crystallography and mutagenesis studies show that mannose is ligated to the conserved Ca2+ in the primary binding site that is characteristic of C-type CRDs, and the GlcNAc and galactose residues make additional interactions in a wide, shallow groove adjacent to the primary binding site. As predicted from these studies, BDCA-2 binds to IgG, which bears galactose-terminated glycans that are not commonly found attached to other serum glycoproteins. Thus, BDCA-2 has the potential to serve as a previously unrecognized immunoglobulin Fc receptor. | |||
A novel mechanism for binding of galactose-terminated glycans by the C-type carbohydrate-recognition domain in blood dendritic cell antigen 2.,Jegouzo SA, Feinberg H, Dungarwalla T, Drickamer K, Weis WI, Taylor ME J Biol Chem. 2015 May 20. pii: jbc.M115.660613. PMID:25995448<ref>PMID:25995448</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4zes" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Drickamer K]] | ||
[[Category: | [[Category: Dungarwalla T]] | ||
[[Category: | [[Category: Feinberg H]] | ||
[[Category: | [[Category: Jegouzo SAF]] | ||
[[Category: | [[Category: Taylor ME]] | ||
[[Category: | [[Category: Weis WI]] | ||
Latest revision as of 11:35, 6 November 2024
Blood dendritic cell antigen 2 (BDCA-2) complexed with methyl-mannoside
| ||||||||||||
