5wjf: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:


==Crystal structure of murine 4-1BB from HEK293T cells in P21212 space group==
==Crystal structure of murine 4-1BB from HEK293T cells in P21212 space group==
<StructureSection load='5wjf' size='340' side='right' caption='[[5wjf]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='5wjf' size='340' side='right'caption='[[5wjf]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5wjf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJF FirstGlance]. <br>
<table><tr><td colspan='2'>[[5wjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WJF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjf OCA], [http://pdbe.org/5wjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjf RCSB], [http://www.ebi.ac.uk/pdbsum/5wjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjf ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjf OCA], [https://pdbe.org/5wjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wjf RCSB], [https://www.ebi.ac.uk/pdbsum/5wjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TNR9_MOUSE TNR9_MOUSE]] Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.  
[https://www.uniprot.org/uniprot/TNR9_MOUSE TNR9_MOUSE] Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
4-1BB (CD137) is a TNF receptor superfamily (TNFRSF) member that is thought to undergo receptor trimerization upon binding to its trimeric TNF superfamily ligand (4-1BBL) to stimulate immune responses. 4-1BB also can bind to the tandem repeat-type lectin galectin-9 (Gal-9), and signaling through mouse (m)4-1BB is reduced in galectin-9 (Gal-9)-deficient mice, suggesting a pivotal role of Gal-9 in m4-1BB activation. Here, using sulfur-SAD phasing, we determined the crystal structure of m4-1BB to 2.2-A resolution. We found that similar to other TNFRSFs, m4-1BB has four cysteine-rich domains (CRDs). However, the organization of CRD1 and the orientation of CRD3 and CRD4 with respect to CRD2 in the m4-1BB structure distinctly differed from those of other TNFRSFs. Moreover, we mapped two Asn residues within CRD4 that are N-linked glycosylated and mediate m4-1BB binding to Gal-9. Kinetics studies of m4-1BB disclosed a very tight nanomolar binding affinity to m4-1BBL with an unexpectedly strong avidity effect. Both N- and C-terminal domains of Gal-9 bound m4-1BB, but with lower affinity compared with m4-1BBL. Although the TNF homology domain (THD) of human (h)4-1BBL forms non-covalent trimers, we found that m4-1BBL formed a covalent dimer via 2 cysteines absent in h4-1BBL. As multimerization and clustering is a prerequisite for TNFR intracellular signaling, and as m4-1BBL can only recruit two m4-1BB monomers, we hypothesize that m4-1BBL and Gal-9 act together to aid aggregation of m4-1BB monomers to efficiently initiate m4-1BB signaling.
 
Crystal structure of murine 4-1BB and its interaction with 4-1BBL support a role for galectin-9 in 4-1BB signaling.,Bitra A, Doukov T, Wang J, Picarda G, Benedict CA, Croft M, Zajonc DM J Biol Chem. 2018 Jan 26;293(4):1317-1329. doi: 10.1074/jbc.M117.814905. Epub, 2017 Dec 14. PMID:29242193<ref>PMID:29242193</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wjf" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Tumor necrosis factor receptor 3D structures|Tumor necrosis factor receptor 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bitra, A]]
[[Category: Large Structures]]
[[Category: Doukov, T]]
[[Category: Mus musculus]]
[[Category: Zajonc, D M]]
[[Category: Bitra A]]
[[Category: Apoptosis]]
[[Category: Doukov T]]
[[Category: Immune system]]
[[Category: Zajonc DM]]
[[Category: Tumor necrosis factor]]
Retrieved from "https://proteopedia.org/5wjf"