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[[Image:1bb7.gif|left|200px]]
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{{STRUCTURE_1bb7|  PDB=1bb7  |  SCENE=  }}
'''LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOBIOSE'''


==LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOBIOSE==
<StructureSection load='1bb7' size='340' side='right'caption='[[1bb7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BB7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GUM:4-METHYL-UMBELLIFERYL-N-ACETYL-CHITOBIOSE'>GUM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bb7 OCA], [https://pdbe.org/1bb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bb7 RCSB], [https://www.ebi.ac.uk/pdbsum/1bb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bb7 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/1bb7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bb7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Two complexes between rainbow trout lysozyme (RBTL) and 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution. The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL. This agrees well with earlier crystallographic studies on the binding of oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white lysozymes. For both complexes the 4-MeU moiety in site D has diffuse electron density and is flexible, as it is only bound to water molecules and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate complexes.


==Overview==
Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme.,Vollan VB, Hough E, Karlsen S Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:10089395<ref>PMID:10089395</ref>
Two complexes between rainbow trout lysozyme (RBTL) and 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution. The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL. This agrees well with earlier crystallographic studies on the binding of oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white lysozymes. For both complexes the 4-MeU moiety in site D has diffuse electron density and is flexible, as it is only bound to water molecules and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate complexes.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1BB7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB7 OCA].
</div>
<div class="pdbe-citations 1bb7" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme., Vollan VB, Hough E, Karlsen S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089395 10089395]
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
[[Category: Lysozyme]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oncorhynchus mykiss]]
[[Category: Oncorhynchus mykiss]]
[[Category: Single protein]]
[[Category: Hough E]]
[[Category: Hough, E.]]
[[Category: Karlsen S]]
[[Category: Karlsen, S.]]
[[Category: Vollan VB]]
[[Category: Vollan, V B.]]
[[Category: Hydrolase]]
[[Category: N-acetyl-muramidase]]
[[Category: Umbelliferone glycoside]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:17:57 2008''

Latest revision as of 23:49, 20 November 2024

LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOBIOSE

1bb7, resolution 2.00Å

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