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[[Image:2ar0.gif|left|200px]]


{{Structure
==Crystal structure of Type I restriction enzyme EcoKI M protein (EC 2.1.1.72) (M.EcoKI)==
|PDB= 2ar0 |SIZE=350|CAPTION= <scene name='initialview01'>2ar0</scene>, resolution 2.80&Aring;
<StructureSection load='2ar0' size='340' side='right'caption='[[2ar0]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=UNX:UNKNOWN ATOM OR ION'>UNX</scene>
<table><tr><td colspan='2'>[[2ar0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AR0 FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
|GENE= hsdM, hsm ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ar0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ar0 OCA], [https://pdbe.org/2ar0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ar0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ar0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ar0 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/2ar0 TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T1MK_ECOLI T1MK_ECOLI] The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoKI enzyme recognizes 5'-AACN(6)GTGC-3'.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/2ar0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ar0 ConSurf].
<div style="clear:both"></div>


'''Crystal structure of Type I restriction enzyme EcoKI M protein (EC 2.1.1.72) (M.EcoKI)'''
==See Also==
 
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==About this Structure==
</StructureSection>
2AR0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AR0 OCA].
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Site-specific DNA-methyltransferase (adenine-specific)]]
[[Category: Burley SK]]
[[Category: Burley, S K.]]
[[Category: Kniewel R]]
[[Category: Kniewel, R.]]
[[Category: Lima CD]]
[[Category: Lima, C D.]]
[[Category: Rajashankar KR]]
[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
[[Category: Rajashankar, K R.]]
[[Category: UNX]]
[[Category: new york structural genomix research consortium]]
[[Category: psi]]
[[Category: structural genomics; protein structure initiative; nysgxrc; t824; q9pnp0 restriction modification enzyme; type i restriction enzyme ecoki m protein; methyltransferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:52:48 2008''
Retrieved from "https://proteopedia.org/2ar0"