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[[Image:2bob.gif|left|200px]]
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{{STRUCTURE_2bob|  PDB=2bob  |  SCENE=  }}
'''POTASSIUM CHANNEL KCSA-FAB COMPLEX IN THALLIUM WITH TETRABUTYLAMMONIUM (TBA)'''


==Potassium channel KcsA-Fab complex in thallium with tetrabutylammonium (TBA)==
<StructureSection load='2bob' size='340' side='right'caption='[[2bob]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bob]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BOB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=TBA:TETRABUTYLAMMONIUM+ION'>TBA</scene>, <scene name='pdbligand=TL:THALLIUM+(I)+ION'>TL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bob OCA], [https://pdbe.org/2bob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bob RCSB], [https://www.ebi.ac.uk/pdbsum/2bob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bob ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/2bob_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bob ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Potassium channels catalyze the selective transfer of potassium across the cell membrane and are essential for setting the resting potential in cells, controlling heart rate and modulating the firing pattern in neurons. Tetraethylammonium (TEA) blocks ion conduction through potassium channels in a voltage-dependent manner from both sides of the membrane. Here we show the structural basis of TEA blockade by cocrystallizing the prokaryotic potassium channel KcsA with two selective TEA analogs. TEA binding at both sites alters ion occupancy in the selectivity filter; these findings underlie the mutual destabilization and voltage-dependence of TEA blockade. We propose that TEA blocks potassium channels by acting as a potassium analog at the dehydration transition step during permeation.


==Overview==
Structural basis of TEA blockade in a model potassium channel.,Lenaeus MJ, Vamvouka M, Focia PJ, Gross A Nat Struct Mol Biol. 2005 May;12(5):454-9. Epub 2005 Apr 24. PMID:15852022<ref>PMID:15852022</ref>
Potassium channels catalyze the selective transfer of potassium across the cell membrane and are essential for setting the resting potential in cells, controlling heart rate and modulating the firing pattern in neurons. Tetraethylammonium (TEA) blocks ion conduction through potassium channels in a voltage-dependent manner from both sides of the membrane. Here we show the structural basis of TEA blockade by cocrystallizing the prokaryotic potassium channel KcsA with two selective TEA analogs. TEA binding at both sites alters ion occupancy in the selectivity filter; these findings underlie the mutual destabilization and voltage-dependence of TEA blockade. We propose that TEA blocks potassium channels by acting as a potassium analog at the dehydration transition step during permeation.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2BOB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOB OCA].
</div>
<div class="pdbe-citations 2bob" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural basis of TEA blockade in a model potassium channel., Lenaeus MJ, Vamvouka M, Focia PJ, Gross A, Nat Struct Mol Biol. 2005 May;12(5):454-9. Epub 2005 Apr 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15852022 15852022]
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Streptomyces lividans]]
[[Category: Streptomyces lividans]]
[[Category: Focia, P J.]]
[[Category: Focia PJ]]
[[Category: Gross, A.]]
[[Category: Gross A]]
[[Category: Lenaeus, M J.]]
[[Category: Lenaeus MJ]]
[[Category: Vamvouka, M.]]
[[Category: Vamvouka M]]
[[Category: Ion transport]]
[[Category: Ionic channel]]
[[Category: Potassium channel]]
[[Category: Protein-antibody fab complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:34:00 2008''
Retrieved from "https://proteopedia.org/2bob"