2cw9: Difference between revisions
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< | ==Crystal structure of human Tim44 C-terminal domain== | ||
<StructureSection load='2cw9' size='340' side='right'caption='[[2cw9]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
You may | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2cw9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CW9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
-- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cw9 OCA], [https://pdbe.org/2cw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cw9 RCSB], [https://www.ebi.ac.uk/pdbsum/2cw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cw9 ProSAT], [https://www.topsan.org/Proteins/RSGI/2cw9 TOPSAN]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cw9_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cw9 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Familial oncocytic thyroid carcinoma is associated with a missense mutation, P308Q, in the C-terminal domain of Tim44. Tim44 is the mitochondrial inner-membrane translocase subunit and it functions as a membrane anchor for the mitochondrial heat-shock protein 70 (mtHsp70). Here, the crystal structure of the human Tim44 C-terminal domain complexed with pentaethylene glycol has been determined at 1.9 A resolution. The overall structure resembles that of the nuclear transport factor 2-like domain. In the crystal structure, pentaethylene glycol molecules are associated at two potential membrane-binding sites: the large hydrophobic cavity and the highly conserved loop between the alpha1 and alpha2 helices near Pro308. A comparison with the yeast homolog revealed that lipid binding induces conformational changes around the alpha1-alpha2 loop, leading to slippage of the alpha1 helix along the large beta-sheet. These changes may play important roles in the translocation of polypeptides across the mitochondrial inner membrane. | |||
Structure of the human Tim44 C-terminal domain in complex with pentaethylene glycol: ligand-bound form.,Handa N, Kishishita S, Morita S, Akasaka R, Jin Z, Chrzas J, Chen L, Liu ZJ, Wang BC, Sugano S, Tanaka A, Terada T, Shirouzu M, Yokoyama S Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1225-34. Epub 2007, Nov 16. PMID:18084070<ref>PMID:18084070</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2cw9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Chen | [[Category: Chen L]] | ||
[[Category: Chrzas | [[Category: Chrzas J]] | ||
[[Category: Handa | [[Category: Handa N]] | ||
[[Category: Jin | [[Category: Jin Z]] | ||
[[Category: Kinoshita | [[Category: Kinoshita Y]] | ||
[[Category: Kishishita | [[Category: Kishishita S]] | ||
[[Category: Liu | [[Category: Liu Z-J]] | ||
[[Category: Morita | [[Category: Morita S]] | ||
[[Category: Nagano | [[Category: Nagano Y]] | ||
[[Category: Shirouzu M]] | |||
[[Category: Shirouzu | [[Category: Takemoto C]] | ||
[[Category: Takemoto | [[Category: Terada T]] | ||
[[Category: Terada | [[Category: Uchikubo T]] | ||
[[Category: Uchikubo | [[Category: Uda H]] | ||
[[Category: Uda | [[Category: Wang B-C]] | ||
[[Category: Wang | [[Category: Yokoyama S]] | ||
[[Category: Yokoyama | |||
Latest revision as of 00:51, 21 November 2024
Crystal structure of human Tim44 C-terminal domain
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