3n0r

Structure of the PhyR stress response regulator at 1.25 Angstrom resolution

spinqualitylabelsall models 3n0r, resolution 1.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3n0r is a 1 chain structure with sequence from Caulobacter vibrioides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.251Å Ligands: GOL, MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9A2S9_CAUVC Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed PhyR is a hybrid stress regulator conserved in alpha-proteobacteria that contains an N-terminal sigma-like (SL) domain and a C-terminal receiver domain. Phosphorylation of the receiver domain is known to promote binding of the SL domain to an anti-sigma factor. PhyR thus functions as an anti-anti-sigma factor in its phosphorylated state. We present genetic evidence that Caulobacter crescentus PhyR is a phosphorylation-dependent stress regulator that functions in the same pathway as sigma(T) and its anti-sigma factor, NepR. Additionally, we report the X-ray crystal structure of PhyR at 1.25 A resolution, which provides insight into the mechanism of anti-anti-sigma regulation. Direct intramolecular contact between the PhyR receiver and SL domains spans regions sigma and sigma, likely serving to stabilize the SL domain in a closed conformation. The molecular surface of the receiver domain contacting the SL domain is the structural equivalent of alpha4-beta5-alpha5, which is known to undergo dynamic conformational change upon phosphorylation in a diverse range of receiver proteins. We propose a structural model of PhyR regulation in which receiver phosphorylation destabilizes the intramolecular interaction between SL and receiver domains, thereby permitting regions sigma and sigma in the SL domain to open about a flexible connector loop and bind anti-sigma factor. A structural model of anti-anti-sigma inhibition by a two-component receiver domain: the PhyR stress response regulator.,Herrou J, Foreman R, Fiebig A, Crosson S Mol Microbiol. 2010 Oct;78(2):290-304. doi:, 10.1111/j.1365-2958.2010.07323.x. Epub 2010 Aug 18. PMID:20735776 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Response regulator 3D structure

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