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{{STRUCTURE_4dwq|  PDB=4dwq  |  SCENE=  }}
===RNA ligase RtcB-GMP/Mn(2+) complex===
{{ABSTRACT_PUBMED_22949672}}


==Function==
==RNA ligase RtcB-GMP/Mn(2+) complex==
[[http://www.uniprot.org/uniprot/RTCB_PYRHO RTCB_PYRHO]] tRNA-splicing ligase that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as a RNA ligase with broad substrate specificity, and may function toward other RNAs (By similarity).  
<StructureSection load='4dwq' size='340' side='right'caption='[[4dwq]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4dwq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DWQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dwq OCA], [https://pdbe.org/4dwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dwq RCSB], [https://www.ebi.ac.uk/pdbsum/4dwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dwq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RTCB_PYRHO RTCB_PYRHO] tRNA-splicing ligase that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as a RNA ligase with broad substrate specificity, and may function toward other RNAs (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The RtcB protein has recently been identified as a 3'-phosphate RNA ligase that directly joins an RNA strand ending with a 2',3'-cyclic phosphate to the 5'-hydroxyl group of another RNA strand in a GTP/Mn(2+)-dependent reaction. Here, we report two crystal structures of Pyrococcus horikoshii RNA-splicing ligase RtcB in complex with Mn(2+) alone (RtcB/ Mn(2+)) and together with a covalently bound GMP (RtcB-GMP/Mn(2+)). The RtcB/ Mn(2+) structure (at 1.6 A resolution) shows two Mn(2+) ions at the active site, and an array of sulfate ions nearby that indicate the binding sites of the RNA phosphate backbone. The structure of the RtcB-GMP/Mn(2+) complex (at 2.3 A resolution) reveals the detailed geometry of guanylylation of histidine 404. The critical roles of the key residues involved in the binding of the two Mn(2+) ions, the four sulfates, and GMP are validated in extensive mutagenesis and biochemical experiments, which also provide a thorough characterization for the three steps of the RtcB ligation pathway: (i) guanylylation of the enzyme, (ii) guanylyl-transfer to the RNA substrate, and (iii) overall ligation. These results demonstrate that the enzyme's substrate-induced GTP binding site and the putative reactive RNA ends are in the vicinity of the binuclear Mn(2+) active center, which provides detailed insight into how the enzyme-bound GMP is tansferred to the 3'-phosphate of the RNA substrate for activation and subsequent nucleophilic attack by the 5'-hydroxyl of the second RNA substrate, resulting in the ligated product and release of GMP.


==About this Structure==
Structural and mechanistic insights into guanylylation of RNA-splicing ligase RtcB joining RNA between 3'-terminal phosphate and 5'-OH.,Englert M, Xia S, Okada C, Nakamura A, Tanavde V, Yao M, Eom SH, Konigsberg WH, Soll D, Wang J Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15235-40. Epub 2012 Sep 4. PMID:22949672<ref>PMID:22949672</ref>
[[4dwq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DWQ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:022949672</ref><references group="xtra"/><references/>
</div>
[[Category: Pyrococcus shinkaii]]
<div class="pdbe-citations 4dwq" style="background-color:#fffaf0;"></div>
[[Category: Englert, M.]]
 
[[Category: Okada, C.]]
==See Also==
[[Category: Soll, D.]]
*[[RNA ligase|RNA ligase]]
[[Category: Wang, J.]]
== References ==
[[Category: Xia, S.]]
<references/>
[[Category: Yao, M.]]
__TOC__
[[Category: Guanylylation]]
</StructureSection>
[[Category: Ligase]]
[[Category: Large Structures]]
[[Category: Mn2+]]
[[Category: Pyrococcus horikoshii]]
[[Category: Rna ligase]]
[[Category: Englert M]]
[[Category: Rtcb]]
[[Category: Okada C]]
[[Category: Rtcb-gmp]]
[[Category: Soll D]]
[[Category: Wang J]]
[[Category: Xia S]]
[[Category: Yao M]]
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