3hl2: Difference between revisions

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{{Seed}}
[[Image:3hl2.jpg|left|200px]]


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==The crystal structure of the human SepSecS-tRNASec complex==
The line below this paragraph, containing "STRUCTURE_3hl2", creates the "Structure Box" on the page.
<StructureSection load='3hl2' size='340' side='right'caption='[[3hl2]], [[Resolution|resolution]] 2.81&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3hl2]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HL2 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLR:(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>PLR</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TS6:MONOTHIOPHOSPHATE'>TS6</scene></td></tr>
{{STRUCTURE_3hl2|  PDB=3hl2  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hl2 OCA], [https://pdbe.org/3hl2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hl2 RCSB], [https://www.ebi.ac.uk/pdbsum/3hl2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hl2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPCS_HUMAN SPCS_HUMAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hl2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hl2 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Selenocysteine is the only genetically encoded amino acid in humans whose biosynthesis occurs on its cognate transfer RNA (tRNA). O-Phosphoseryl-tRNA:selenocysteinyl-tRNA synthase (SepSecS) catalyzes the final step of selenocysteine formation by a poorly understood tRNA-dependent mechanism. The crystal structure of human tRNA(Sec) in complex with SepSecS, phosphoserine, and thiophosphate, together with in vivo and in vitro enzyme assays, supports a pyridoxal phosphate-dependent mechanism of Sec-tRNA(Sec) formation. Two tRNA(Sec) molecules, with a fold distinct from other canonical tRNAs, bind to each SepSecS tetramer through their 13-base pair acceptor-TPsiC arm (where Psi indicates pseudouridine). The tRNA binding is likely to induce a conformational change in the enzyme's active site that allows a phosphoserine covalently attached to tRNA(Sec), but not free phosphoserine, to be oriented properly for the reaction to occur.


===The crystal structure of the human SepSecS-tRNASec complex===
The human SepSecS-tRNASec complex reveals the mechanism of selenocysteine formation.,Palioura S, Sherrer RL, Steitz TA, Soll D, Simonovic M Science. 2009 Jul 17;325(5938):321-5. PMID:19608919<ref>PMID:19608919</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3hl2" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_19608919}}, adds the Publication Abstract to the page
*[[Selenocysteine synthase|Selenocysteine synthase]]
(as it appears on PubMed at http://www.pubmed.gov), where 19608919 is the PubMed ID number.
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
-->
== References ==
{{ABSTRACT_PUBMED_19608919}}
<references/>
 
__TOC__
==About this Structure==
</StructureSection>
3HL2 is a 5 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HL2 OCA].
 
==Reference==
<ref group="xtra">PMID:19608919</ref><references group="xtra"/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Palioura, S.]]
[[Category: Large Structures]]
[[Category: Simonovic, M]]
[[Category: Palioura S]]
[[Category: Soll, D.]]
[[Category: Simonovic M]]
[[Category: Steitz, T A.]]
[[Category: Soll D]]
[[Category: Alternative splicing]]
[[Category: Steitz TA]]
[[Category: Cytoplasm]]
[[Category: Protein biosynthesis]]
[[Category: Protein-rna complex]]
[[Category: Pyridoxal phosphate]]
[[Category: Selenium]]
[[Category: Selenocysteine]]
[[Category: Sepsec]]
[[Category: Transferase]]
[[Category: Trnasec]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct  7 13:58:10 2009''
Retrieved from "https://proteopedia.org/3hl2"