4itq: Difference between revisions
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==Crystal structure of hypothetical protein SCO1480 bound to DNA== | ==Crystal structure of hypothetical protein SCO1480 bound to DNA== | ||
<StructureSection load='4itq' size='340' side='right' caption='[[4itq]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='4itq' size='340' side='right'caption='[[4itq]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4itq]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4itq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ITQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ITQ FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4itq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4itq OCA], [https://pdbe.org/4itq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4itq RCSB], [https://www.ebi.ac.uk/pdbsum/4itq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4itq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/IHF_STRCO IHF_STRCO] A nucleoid-associated protein (NAP) that probably plays a role in chromosome compactation. Contributes to development and secondary metabolism, but is dispensable for growth and viability (PubMed:22038127, PubMed:23427309). Binds to the promoter region of a number of genes (including itself); multiple molecules of the protein bind to the DNA simultaneously, deletion alters the expression of about 30 genes (both up- and down-regulation occurs). Plays a role in controlling viability (PubMed:22038127). Binds dsDNA without any obvious sequence specificity, in a concentration and length-dependent manner. Promotes supercoiling in a topoisomerase-dependent manner (counteracts TopA plasmid relaxation). Binds DNA as a monomer, contacting 8 base pairs via the phosphate backbone; each monomer can bind 2 DNA duplexes, allowing a bridging function (PubMed:23427309). Alters DNA topology, constraining negative supercoils, possibly by DNA twist. Longer dsDNA binds more than one sIHF subunit (PubMed:31376411).<ref>PMID:22038127</ref> <ref>PMID:23427309</ref> <ref>PMID:31376411</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Elliot | [[Category: Elliot MA]] | ||
[[Category: Gloyd | [[Category: Gloyd M]] | ||
[[Category: Guarne | [[Category: Guarne A]] | ||
[[Category: Nanji | [[Category: Nanji T]] | ||
[[Category: Swiercz | [[Category: Swiercz JP]] | ||
