Sand box 326: Difference between revisions
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Using SwissDock, it was determined that larger, cyclic molecules are good substrates that would have the best binding affinity to 3CBW, each had about a -7 kcal/mol binding affinity. These large molecules are 4-Nitrophenyl N-acetyl-β-D-glucosaminide, 4-Nitrophenyl α-D-glucopyranoside, and p-nitrophenyl phosphate. | Using SwissDock, it was determined that larger, cyclic molecules are good substrates that would have the best binding affinity to 3CBW, each had about a -7 kcal/mol binding affinity. These large molecules are 4-Nitrophenyl N-acetyl-β-D-glucosaminide, 4-Nitrophenyl α-D-glucopyranoside, and p-nitrophenyl phosphate. | ||
== Bradford Assay Results == | |||
3CBW is catalytically active at a higher temperature (70 ºC) when reacted with p-nitrophenol(PNP). | |||
[[Image:Screenshot_2025-04-28_110316.png |300px|center|thumb|]] | |||
== Theoretical Functionality and Proposed Bodily Purpose == | == Theoretical Functionality and Proposed Bodily Purpose == | ||
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A) Dhawan, S.; Kaur, J.; Microbial Mannanases: An Overview of Production and Applications. Critical Reviews in Biotechnology 2007, 27, 197-216. DOI: 10.1080/07388550701775919 | A) Dhawan, S.; Kaur, J.; Microbial Mannanases: An Overview of Production and Applications. Critical Reviews in Biotechnology 2007, 27, 197-216. DOI: 10.1080/07388550701775919 | ||
B) Soni, H.; Rawat, H. K.; Pletschke, B. I.; Kango, N. Purification and characterization of Beta-mannanase from Aspergillus terreus and its applicability in depolymerization of mannans and saccharification of lignocellulosic biomass. Biotech 2016, 6, 136. DOI: 10.1007/s13205-016-0454-2 | B) Soni, H.; Rawat, H. K.; Pletschke, B. I.; Kango, N. Purification and characterization of Beta-mannanase from Aspergillus terreus and its applicability in depolymerization of mannans and saccharification of lignocellulosic biomass. Biotech 2016, 6, 136. DOI: 10.1007/s13205-016-0454-2 | ||
C) Cheng, L.; Duan, S.; Feng, X.; Zheng, K.; Yang, Q.; Liu, Z. Purification and Characterization of a Thermostable Beta-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International 2016, 2016, 1-7. DOI: 10.1155/2016/6380147 | C) Cheng, L.; Duan, S.; Feng, X.; Zheng, K.; Yang, Q.; Liu, Z. Purification and Characterization of a Thermostable Beta-Mannanase from Bacillus subtilis BE-91: Potential Application in Inflammatory Diseases. BioMed Research International 2016, 2016, 1-7. DOI: 10.1155/2016/6380147 | ||
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