7lnk: Difference between revisions
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New page: '''Unreleased structure''' The entry 7lnk is ON HOLD Authors: Yu, J.C., Mietzsch, M., Agbandje-McKenna, M. Description: Gorilla Bocavirus 1 Capsid Category: Unreleased Structures [... |
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==Gorilla Bocavirus 1 Capsid== | |||
<StructureSection load='7lnk' size='340' side='right'caption='[[7lnk]], [[Resolution|resolution]] 2.76Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LNK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.76Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lnk OCA], [https://pdbe.org/7lnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lnk RCSB], [https://www.ebi.ac.uk/pdbsum/7lnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lnk ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Human bocavirus 1 (HBoV1) has gained attention as a gene delivery vector with its ability to infect polarized human airway epithelia and 5.5 kb genome packaging capacity. Gorilla bocavirus 1 (GBoV1) VP3 shares 86% amino acid sequence identity with HBoV1 but has better transduction efficiency in several human cell types. Here, we report the capsid structure of GBoV1 determined to 2.76 A resolution using cryo-electron microscopy (cryo-EM) and its interaction with mouse monoclonal antibodies (mAbs) and human sera. GBoV1 shares capsid surface morphologies with other parvoviruses, with a channel at the 5-fold symmetry axis, protrusions surrounding the 3-fold axis and a depression at the 2-fold axis. A 2/5-fold wall separates the 2-fold and 5-fold axes. Compared to HBoV1, differences are localized to the 3-fold protrusions. Consistently, native dot immunoblots and cryo-EM showed cross-reactivity and binding, respectively, by a 5-fold targeted HBoV1 mAb, 15C6. Surprisingly, recognition was observed for one out of three 3-fold targeted mAbs, 12C1, indicating some structural similarity at this region. In addition, GBoV1, tested against 40 human sera, showed the similar rates of seropositivity as HBoV1. Immunogenic reactivity against parvoviral vectors is a significant barrier to efficient gene delivery. This study is a step towards optimizing bocaparvovirus vectors with antibody escape properties. | |||
Characterization of the GBoV1 Capsid and Its Antibody Interactions.,Yu JC, Mietzsch M, Singh A, Jimenez Ybargollin A, Kailasan S, Chipman P, Bhattacharya N, Fakhiri J, Grimm D, Kapoor A, Kucinskaite-Kodze I, Zvirbliene A, Soderlund-Venermo M, McKenna R, Agbandje-McKenna M Viruses. 2021 Feb 20;13(2). pii: v13020330. doi: 10.3390/v13020330. PMID:33672786<ref>PMID:33672786</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7lnk" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Agbandje-McKenna M]] | |||
[[Category: Mietzsch M]] | |||
[[Category: Yu JC]] | |||
