Isopenicillin N synthase: Difference between revisions
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Michal Harel (talk | contribs) New page: == Your Heading Here (maybe something like 'Structure') == <StructureSection load='1dq8' size='350' side='right' caption='Structure of HMG-CoA reductase (PDB entry 1dq8)' scene=''> Any... |
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<StructureSection load='1blz' size='350' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'> | |||
<StructureSection load=' | |||
== Function == | |||
'''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O. | '''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>. | ||
== Structural highlights == | |||
IPNS <scene name='52/525170/Cv/7'>active site contains Fe+2 and the substrate ACV</scene>. The <scene name='52/525170/Cv/8'>Fe+2 ion is hexacoordinated to 3 IPNS side chains, one water molecule, the ACV thiolate moiety and NO</scene><ref>PMID:9194566</ref>. Water molecules shown as red spheres. | |||
</StructureSection> | |||
==3D structures of isopenicillin N synthase== | ==3D structures of isopenicillin N synthase== | ||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | |||
{{#tree:id=OrganizedByTopic|openlevels=0| | |||
*IPNS | |||
[[ | **[[8bbb]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | ||
[[ | **[[6jyv]] – IPNS – ''Pseudomonas aeruginosa''<br /> | ||
*Fe containing IPNS | |||
[[1ips]] – AnIPNS | **[[1bk0]], [[6y0o]], [[6zae]], [[6zaf]], [[6zag]], [[6zah]], [[6zam]] - AnIPNS + ACV + Fe <br /> | ||
**[[2bjs]], [[7zoe]], [[8alj]], [[8bb9]], [[8bba]], [[8bbd]], [[8bsv]], [[8bsy]], [[8p47]], [[8ptv]] - AnIPNS (mutant) + ACV + Fe<br /> | |||
**[[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1oc1]], [[1odm]], [[1odn]], [[1qiq]], [[1qjf]], [[1uzw]], [[1w03]], [[1w05]], [[1w3v]], [[1w3x]], [[2bu9]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y60]], [[2y6f]], [[4bb3]], [[8p7p]], [[8py5]] - AnIPNS + ACV derivative + Fe<br /> | |||
**[[8py8]], [[8py9]] - AnIPNS + ACV derivative + Fe + O2<br /> | |||
**[[2jb4]] - AnIPNS + cyclobutanone analog + Fe<br /> | |||
**[[6y0p]], [[6zao]], [[6zaq]], [[8py6]] - AnIPNS + IPN + Fe<br /> | |||
**[[8p7o]] - AnIPNS + inhibitor + Fe<br /> | |||
**[[7p3l]] - AnIPNS + substate analog + Fe<br /> | |||
**[[8p46]] - AnIPNS (mutant) + IPN + Fe<br /> | |||
**[[1qje]] - AnIPNS + IPN + ACV<br /> | |||
**[[6zai]], [[6zaj]], [[6zal]], [[6zap]] - AnIPNS + O2 + ACV<br /> | |||
**[[7poy]] - AnIPNS (mutant) + NO + ACV<br /> | |||
**[[8py7]], [[8pya]] - AnIPNS (mutant) + Fe + O2 + ACV<br /> | |||
**[[7psw]], [[8ali]], [[8bbc]], [[8bsw]], [[8bsx]], [[8p48]], [[8rdb]] - AnIPNS (mutant) + Fe + ACV<br /> | |||
**[[1blz]], [[6zan]] - AnIPNS + NO + ACV<br /> | |||
**[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | |||
**[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | |||
*Other metal containing IPNS | |||
**[[1ips]] – AnIPNS + Mn<br /> | |||
**[[6zw8]] - AnIPNS + ACV + Cd<br /> | |||
**[[8a4g]] - AnIPNS (mutant) + ACV + Cd<br /> | |||
**[[6zAK]] - AnIPNS + ACV + VO2<br /> | |||
}} | |||
== References == | |||
<references/> | |||
[[Category: Topic Page]] | |||
