Isopenicillin N synthase: Difference between revisions

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{{STRUCTURE_1blz|  PDB=1blz | SIZE=400| SCENE= |right|CAPTION=Isopenicilln N synthase complex with ACV and NO, [[1blz]] }}
<StructureSection load='1blz' size='350' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'>
 
== Function ==
== Function ==
'''Isopenicillin N synthase''' (IPNS) is an iron-dependent  enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV).  IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics.  The active site of IPNS contains an Fe atom.  The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>.
'''Isopenicillin N synthase''' (IPNS) is an iron-dependent  enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV).  IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics.  The active site of IPNS contains an Fe atom.  The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>.


== Structural highlights ==
== Structural highlights ==
IPNS active site contains Fe+2 and the substrate ACV. The Fe+2 ion is pentacoordinated to 3 IPNS side chains, one water molecule and the ACV thiolate moiety<ref>PMID:9194566</ref>.
IPNS <scene name='52/525170/Cv/7'>active site contains Fe+2 and the substrate ACV</scene>. The <scene name='52/525170/Cv/8'>Fe+2 ion is hexacoordinated to 3 IPNS side chains, one water molecule, the ACV thiolate moiety and NO</scene><ref>PMID:9194566</ref>. Water molecules shown as red spheres.
 
</StructureSection>
==3D structures of isopenicillin N synthase==
==3D structures of isopenicillin N synthase==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
{{#tree:id=OrganizedByTopic|openlevels=0|
{{#tree:id=OrganizedByTopic|openlevels=0|
*IPNS
**[[8bbb]] - AnIPNS + ACV – ''Aspergillus nidulans''<br />
**[[6jyv]] – IPNS – ''Pseudomonas aeruginosa''<br />


*Fe containing IPNS
*Fe containing IPNS


**[[1bk0]] - AnIPNS + ACV – ''Aspergillus nidulans''<br />
**[[1bk0]], [[6y0o]], [[6zae]], [[6zaf]], [[6zag]], [[6zah]], [[6zam]] - AnIPNS + ACV + Fe <br />
**[[2bjs]] - AnIPNS (mutant) + ACV<br />
**[[2bjs]], [[7zoe]], [[8alj]], [[8bb9]], [[8bba]], [[8bbd]], [[8bsv]], [[8bsy]], [[8p47]], [[8ptv]] - AnIPNS (mutant) + ACV + Fe<br />
**[[1qiq]], [[1qjf]], [[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1odm]], [[1odn]], [[1oc1]], [[1uzw]], [[1w03]], [[1w05]], [[2bu9]], [[1w3v]], [[1w3x]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y6f]], [[2y60]], [[2y86]], [[4bb3]] - AnIPNS + ACV derivative<br />
**[[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1oc1]], [[1odm]], [[1odn]], [[1qiq]], [[1qjf]], [[1uzw]], [[1w03]], [[1w05]], [[1w3v]], [[1w3x]], [[2bu9]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y60]], [[2y6f]], [[4bb3]], [[8p7p]], [[8py5]] - AnIPNS + ACV derivative + Fe<br />
**[[2jb4]] - AnIPNS + cyclobutanone analog<br />
**[[8py8]], [[8py9]] - AnIPNS + ACV derivative + Fe + O2<br />
**[[2jb4]] - AnIPNS + cyclobutanone analog + Fe<br />
**[[6y0p]], [[6zao]], [[6zaq]], [[8py6]] - AnIPNS + IPN + Fe<br />
**[[8p7o]] - AnIPNS + inhibitor + Fe<br />
**[[7p3l]] - AnIPNS + substate analog + Fe<br />
**[[8p46]] - AnIPNS (mutant) + IPN + Fe<br />
**[[1qje]] - AnIPNS + IPN + ACV<br />
**[[1qje]] - AnIPNS + IPN + ACV<br />
**[[1blz]] - AnIPNS + NO + ACV<br />
**[[6zai]], [[6zaj]], [[6zal]], [[6zap]] - AnIPNS + O2 + ACV<br />
**[[7poy]] - AnIPNS (mutant) + NO + ACV<br />
**[[8py7]], [[8pya]] - AnIPNS (mutant) + Fe + O2 + ACV<br />
**[[7psw]], [[8ali]], [[8bbc]], [[8bsw]], [[8bsx]], [[8p48]], [[8rdb]] - AnIPNS (mutant) + Fe + ACV<br />
**[[1blz]], [[6zan]] - AnIPNS + NO + ACV<br />
**[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br />
**[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br />
**[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br />
**[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br />


*Mn containing IPNS
*Other metal containing IPNS
 
**[[1ips]] – AnIPNS + Mn<br />
**[[6zw8]] - AnIPNS + ACV + Cd<br />
**[[8a4g]] - AnIPNS (mutant) + ACV + Cd<br />
**[[6zAK]] - AnIPNS + ACV + VO2<br />


**[[1ips]] – AnIPNS
}}
}}
== References ==
== References ==
<references/>
<references/>
[[Category: Topic Page]]
[[Category: Topic Page]]

Latest revision as of 06:45, 8 October 2025

Isopenicilln N synthase complex with ACV and NO, 1blz

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3D structures of isopenicillin N synthase

Updated on 08-October-2025

References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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