Theoretical models: Difference between revisions

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The success of structure prediction methods is assessed biannually in the ''Critical Assessment of techniques for protein Structure Prediction'' ([[CASP]]) competitions<ref>[http://predictioncenter.gc.ucdavis.edu/ Critical Assessment of techniques for protein Structure Prediction (CASP)].</ref>. Crystallographers submit sequences which they have solved, but for which the structures have not yet been published. Modelers predict the structures which are then compared with subsequently published structures. Beginning in CASP5 (2002), the ability to predict [[Intrinsically Disordered Protein|intrinsic disorder]] was included<ref>PMID: 19774619</ref>. Assessment of CASP results is done in a '''double-blind''' manner: the predictors do not have access to the empirical structures, and the assessors do not know the identities of the predictors, which are coded.

There ~~are~~ also competitions to predict protein-protein docking interactions<ref>[http://www.ebi.ac.uk/msd-srv/capri/ CAPRI: Critical Assessment of PRediction of Interactions].</ref>

There have also been competitions to predict protein-protein docking interactions<ref>[http://www.ebi.ac.uk/msd-srv/capri/ CAPRI: Critical Assessment of PRediction of Interactions].</ref>. More recently, AlphaFold Multimer and AlphaFold 3 attempt to predict protein oligomers.

===2024 CASP 16===

Overall, on all fronts, AF3's modeling capabilities are at or close to the state of the art.<ref name="abriata" /> Prediction of protein oligomer complex assemblies "remains an unsolved challenge."<ref>PMID: 41170922</ref>

AlphaFold 2 & 3 have largely solved prediction of protein monomers and domains, "with barely any space for further improvements at the backbone level except for very specific details, irregular secondary structures, and mutational effects that remain challenging to predict."<ref name="abriata">PMID: 41088961</ref> For prediction of protein oligomer assemblies, AlphaFold-based methods "show progress, though complex topologies and in particular antibody-antigen interactions are still difficult. Notably, a priori knowledge of stoichiometry significantly aids assembly prediction. Protein-'''ligand''' co-folding with AF3 demonstrated strong potential for pose prediction, outperforming many participants and some dedicated docking tools in baseline tests, but several caveats hold as discussed. Ligand '''affinity''' prediction is totally unreliable. Nucleic acid structure prediction lags considerably ...."<ref name="abriata" />

===2022: CASP 15===

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==See Also==

*[[AlphaFold/Index]], a list of pages in Proteopedia about Alphafold.

*[[Calculating GDT TS]]

* Theoretical models displayed in Proteopedia must be clearly identified: see [[Proteopedia:Policy#Theoretical Models]] using methods explained at [[Proteopedia:Cookbook#Theoretical Models]].

Theoretical models: Difference between revisions

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@@ Line 51: / Line 51: @@
 The success of structure prediction methods is assessed biannually in the ''Critical Assessment of techniques for protein Structure Prediction'' ([[CASP]]) competitions<ref>[http://predictioncenter.gc.ucdavis.edu/ Critical Assessment of techniques for protein Structure Prediction (CASP)].</ref>. Crystallographers submit sequences which they have solved, but for which the structures have not yet been published. Modelers predict the structures which are then compared with subsequently published structures. Beginning in CASP5 (2002), the ability to predict [[Intrinsically Disordered Protein|intrinsic disorder]] was included<ref>PMID: 19774619</ref>. Assessment of CASP results is done in a '''double-blind''' manner: the predictors do not have access to the empirical structures, and the assessors do not know the identities of the predictors, which are coded.
-There are also competitions to predict protein-protein docking interactions<ref>[http://www.ebi.ac.uk/msd-srv/capri/ CAPRI: Critical Assessment of PRediction of Interactions].</ref>
+There have also been competitions to predict protein-protein docking interactions<ref>[http://www.ebi.ac.uk/msd-srv/capri/ CAPRI: Critical Assessment of PRediction of Interactions].</ref>. More recently, AlphaFold Multimer and AlphaFold 3 attempt to predict protein oligomers.
+===2024 CASP 16===
+Overall, on all fronts, AF3's modeling capabilities are at or close to the state of the art.<ref name="abriata" /> Prediction of protein oligomer complex assemblies "remains an unsolved challenge."<ref>PMID: 41170922</ref>
+AlphaFold 2 & 3 have largely solved prediction of protein monomers and domains, "with barely any space for further improvements at the backbone level except for very specific details, irregular secondary structures, and mutational effects that remain challenging to predict."<ref name="abriata">PMID: 41088961</ref> For prediction of protein oligomer assemblies, AlphaFold-based methods "show progress, though complex topologies and in particular antibody-antigen interactions are still difficult. Notably, a priori knowledge of stoichiometry significantly aids assembly prediction. Protein-'''ligand''' co-folding with AF3 demonstrated strong potential for pose prediction, outperforming many participants and some dedicated docking tools in baseline tests, but several caveats hold as discussed. Ligand '''affinity''' prediction is totally unreliable. Nucleic acid structure prediction lags considerably ...."<ref name="abriata" />
 ===2022: CASP 15===
@@ Line 147: / Line 152: @@
 ==See Also==
+*[[AlphaFold/Index]], a list of pages in Proteopedia about Alphafold.
 *[[Calculating GDT TS]]
 * Theoretical models displayed in Proteopedia must be clearly identified: see [[Proteopedia:Policy#Theoretical Models]] using methods explained at [[Proteopedia:Cookbook#Theoretical Models]].