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==X-ray study of the DNA oligomer d(ATATAT) in P65 space group==
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<StructureSection load='1rsb' size='340' side='right'caption='[[1rsb]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
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<table><tr><td colspan='2'>[[1rsb]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RSB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rsb OCA], [https://pdbe.org/1rsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rsb RCSB], [https://www.ebi.ac.uk/pdbsum/1rsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rsb ProSAT]</span></td></tr>
{{STRUCTURE_1rsb|  PDB=1rsb |  SCENE= }}
</table>
 
<div style="background-color:#fffaf0;">
'''X-ray study of the DNA oligomer d(ATATAT) in P65 space group'''
== Publication Abstract from PubMed ==
 
 
==Overview==
We present and analyze the structure of the oligonucleotide d(ATATAT) found in two different forms by X-ray crystallography and in solution by NMR. We find that in both crystal lattices the oligonucleotide forms an antiparallel double helical duplex in which base pairing is of the Hoogsteen type. The double helix is apparently very similar to the standard B-form of DNA, with about 10 base pairs per turn. However, the adenines in the duplex are flipped over; as a result, the physicochemical features of both grooves of the helix are changed. In particular, the minor groove is narrow and hydrophobic. On the other hand, d(ATATAT) displays a propensity to adopt the B conformation in solution. These results confirm the polymorphism of AT-rich sequences in DNA. Furthermore, we show that extrahelical adenines and thymines can be minor groove binders in Hoogsteen DNA.
We present and analyze the structure of the oligonucleotide d(ATATAT) found in two different forms by X-ray crystallography and in solution by NMR. We find that in both crystal lattices the oligonucleotide forms an antiparallel double helical duplex in which base pairing is of the Hoogsteen type. The double helix is apparently very similar to the standard B-form of DNA, with about 10 base pairs per turn. However, the adenines in the duplex are flipped over; as a result, the physicochemical features of both grooves of the helix are changed. In particular, the minor groove is narrow and hydrophobic. On the other hand, d(ATATAT) displays a propensity to adopt the B conformation in solution. These results confirm the polymorphism of AT-rich sequences in DNA. Furthermore, we show that extrahelical adenines and thymines can be minor groove binders in Hoogsteen DNA.


==About this Structure==
X-ray and NMR studies of the DNA oligomer d(ATATAT): Hoogsteen base pairing in duplex DNA.,Abrescia NG, Gonzalez C, Gouyette C, Subirana JA Biochemistry. 2004 Apr 13;43(14):4092-100. PMID:15065851<ref>PMID:15065851</ref>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RSB OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
X-ray and NMR studies of the DNA oligomer d(ATATAT): Hoogsteen base pairing in duplex DNA., Abrescia NG, Gonzalez C, Gouyette C, Subirana JA, Biochemistry. 2004 Apr 13;43(14):4092-100. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15065851 15065851]
</div>
[[Category: Abrescia, N G.]]
<div class="pdbe-citations 1rsb" style="background-color:#fffaf0;"></div>
[[Category: Gonzalez, C.]]
== References ==
[[Category: Gouyette, C.]]
<references/>
[[Category: Subirana, J A.]]
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[[Category: Antiparallel hoogsteen dna]]
</StructureSection>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:51:13 2008''
[[Category: Large Structures]]
[[Category: Abrescia NG]]
[[Category: Gonzalez C]]
[[Category: Gouyette C]]
[[Category: Subirana JA]]
Retrieved from "https://proteopedia.org/1rsb"