8e8w: Difference between revisions
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New page: '''Unreleased structure''' The entry 8e8w is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 mononuclear Fe(II) structure on the HDO cofactor assembly pathway== | |||
<StructureSection load='8e8w' size='340' side='right'caption='[[8e8w]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8e8w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_achromogenes_subsp._streptozoticus Streptomyces achromogenes subsp. streptozoticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E8W FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e8w OCA], [https://pdbe.org/8e8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e8w RCSB], [https://www.ebi.ac.uk/pdbsum/8e8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e8w ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SZNF_STRC2 SZNF_STRC2] Involved in the biosynthesis of the glucosamine-nitrosourea antibiotic streptozotocin (SZN) (PubMed:30728519, PubMed:30763082). Catalyzes a complex multi-step reaction: the overall reaction is an oxidative rearrangement of the guanidine group of N(omega)-methyl-L-arginine (L-NMA), generating an N-nitrosourea product (PubMed:30728519, PubMed:30763082, PubMed:32511919). SznF first hydroxylates L-NMA to form N(delta)-hydroxy-N(omega)-methyl-L-arginine (L-HMA), which is further hydroxylated to give N(delta)-hydroxy-N(omega)-hydroxy-N(omega)-methyl-L-arginine (L-DHMA) (PubMed:30728519, PubMed:32511919). Subsequently, an oxidative rearrangement converts this intermediate to N(delta)-hydroxy-N(omega)-methyl-N(omega)-nitroso-L-citrulline (PubMed:30728519, PubMed:34004115). This product is unstable, and degrades non-enzymically into nitric oxide and the denitrosated product N(delta)-hydroxy-N(omega)-methyl-L-citrulline (PubMed:30728519).<ref>PMID:30728519</ref> <ref>PMID:30763082</ref> <ref>PMID:32511919</ref> <ref>PMID:34004115</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces achromogenes subsp. streptozoticus]] | |||
[[Category: Boal AK]] | |||
[[Category: McBride MJ]] | |||
Latest revision as of 09:36, 4 March 2026
Crystal structure of SznF from Streptomyces achromogenes var. streptozoticus NRRL 2697 mononuclear Fe(II) structure on the HDO cofactor assembly pathway
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