4dkj: Difference between revisions
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==CpG specific methyltransferase in complex with target DNA== | |||
<StructureSection load='4dkj' size='340' side='right'caption='[[4dkj]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4dkj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Malacoplasma_penetrans_HF-2 Malacoplasma penetrans HF-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DKJ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=C37:5-FLUORO-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>C37</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dkj OCA], [https://pdbe.org/4dkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dkj RCSB], [https://www.ebi.ac.uk/pdbsum/4dkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dkj ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8EVR5_MALP2 Q8EVR5_MALP2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cytosine methylation promotes deamination. In eukaryotes, CpG methylation is thought to account for CpG underrepresentation. Whether scarcity of CpGs in prokaryotic genomes is diagnostic for methylation is not clear. Here, we report that Mycoplasms tend to be CpG depleted and to harbor a family of constitutively expressed or phase variable CpG-specific DNA methyltransferases. The very CpG poor Mycoplasma penetrans and its constitutively active CpG-specific methyltransferase M.MpeI were chosen for further characterization. Genome-wide sequencing of bisulfite-converted DNA indicated that M.MpeI methylated CpG target sites both in vivo and in vitro in a locus-nonselective manner. A crystal structure of M.MpeI with DNA at 2.15-A resolution showed that the substrate base was flipped and that its place in the DNA stack was taken by a glutamine residue. A phenylalanine residue was intercalated into the "weak" CpG step of the nonsubstrate strand, indicating mechanistic similarities in the recognition of the short CpG target sequence by prokaryotic and eukaryotic DNA methyltransferases. | |||
CpG underrepresentation and the bacterial CpG-specific DNA methyltransferase M.MpeI.,Wojciechowski M, Czapinska H, Bochtler M Proc Natl Acad Sci U S A. 2012 Dec 17. PMID:23248272<ref>PMID:23248272</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | <div class="pdbe-citations 4dkj" style="background-color:#fffaf0;"></div> | ||
[[ | == References == | ||
[[Category: | <references/> | ||
[[Category: Bochtler | __TOC__ | ||
[[Category: Czapinska | </StructureSection> | ||
[[Category: Wojciechowski | [[Category: Large Structures]] | ||
[[Category: Malacoplasma penetrans HF-2]] | |||
[[Category: Bochtler M]] | |||
[[Category: Czapinska H]] | |||
[[Category: Wojciechowski M]] | |||
Latest revision as of 13:21, 1 July 2026
CpG specific methyltransferase in complex with target DNA
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