9wsz: Difference between revisions
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==CryoEM structure of baseplate iris structure in the contracted AlgoCIS== | |||
<StructureSection load='9wsz' size='340' side='right'caption='[[9wsz]], [[Resolution|resolution]] 3.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9wsz]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Algoriphagus_machipongonensis Algoriphagus machipongonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WSZ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wsz OCA], [https://pdbe.org/9wsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wsz RCSB], [https://www.ebi.ac.uk/pdbsum/9wsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wsz ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A3HTB3_9BACT A3HTB3_9BACT] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Contractile injection systems (CISs) mediate cell-cell interactions and are widespread among bacteria and archaea. These phage tail-like macromolecular machines puncture their target by a tube that is propelled by a contractile sheath. The mechanism underlying CIS firing, which starts with target binding and ends with sheath contraction, remains unclear. Here, we investigate the extracellular CIS from Algoriphagus machipongonensis (AlgoCIS) by a multimodal cryo-electron microscopy approach and structure-guided engineering, which allowed us to arrest AlgoCIS in multiple intermediate states of firing. Together with the post-firing structure, our data suggest a stepwise firing mechanism involving all structural modules: signal propagation starts with the binding of the tail-fibers, followed by opening of the cage, an expansion of the baseplate iris, and resulting in sheath contraction and the release of cap adaptor. Our study will serve as a framework for understanding the firing mechanism of diverse CISs and will facilitate the engineering of CISs for biomedical applications. | |||
Stepwise firing mechanism of an extracellular contractile injection system.,Xu J, Ericson CF, Toenshoff ER, Pilhofer M Nat Commun. 2026 Apr 24. doi: 10.1038/s41467-026-72240-y. PMID:42031783<ref>PMID:42031783</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9wsz" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Algoriphagus machipongonensis]] | |||
[[Category: Large Structures]] | |||
[[Category: Ericson CF]] | |||
[[Category: Pilhofer M]] | |||
[[Category: Toenshoff ER]] | |||
[[Category: Xu J]] | |||
Latest revision as of 07:38, 15 July 2026
CryoEM structure of baseplate iris structure in the contracted AlgoCIS
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