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New page: left|200px<br /><applet load="1fer" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fer, resolution 2.3Å" /> '''STRUCTURE AT PH 6.5 O...
 
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[[Image:1fer.jpg|left|200px]]<br /><applet load="1fer" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fer, resolution 2.3&Aring;" />
'''STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION'''<br />


==Overview==
==STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION==
Ferredoxin I from Azotobacter vinelandii (AvFdI) is an iron-sulfur protein, composed of 106 amino acids, seven Fe atoms and eight inorganic S* atoms., A crystallographic redetermination of its structure showed the originally, reported structure to be incorrect. We report here the crystal structure, of AvFdI at pH 6.5. Extensive refinement has led to a final R value of, 0.170 for all 6986 non-extinct reflections in the range 10-2.3 A using a, solvent model which includes 98 discrete solvent atoms with occupancies, between 0.3 and 1.0 and an average B value of 22.5 A(2). The first half of, the peptide chain closely resembles that of the 55-residue ferredoxin from, Peptococcus aerogenes (PaFd), while the remainder consists of three turns, of helix and a series of loops which form a cap over part of the molecular, core. Despite the similarities in structure and surroundings, the, corresponding 4Fe4S* clusters in PaFd and AvFdI have strikingly different, redox potentials; a possible explanation has been sought in the differing, hydration models for the two molecules.
<StructureSection load='1fer' size='340' side='right'caption='[[1fer]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1fer]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FER FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fer OCA], [https://pdbe.org/1fer PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fer RCSB], [https://www.ebi.ac.uk/pdbsum/1fer PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fer ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FER1_AZOVI FER1_AZOVI] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. This ferredoxin could play a role in regulating gene expression by interacting directly with DNA.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/1fer_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fer ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ferredoxin I from Azotobacter vinelandii (AvFdI) is an iron-sulfur protein composed of 106 amino acids, seven Fe atoms and eight inorganic S* atoms. A crystallographic redetermination of its structure showed the originally reported structure to be incorrect. We report here the crystal structure of AvFdI at pH 6.5. Extensive refinement has led to a final R value of 0.170 for all 6986 non-extinct reflections in the range 10-2.3 A using a solvent model which includes 98 discrete solvent atoms with occupancies between 0.3 and 1.0 and an average B value of 22.5 A(2). The first half of the peptide chain closely resembles that of the 55-residue ferredoxin from Peptococcus aerogenes (PaFd), while the remainder consists of three turns of helix and a series of loops which form a cap over part of the molecular core. Despite the similarities in structure and surroundings, the corresponding 4Fe4S* clusters in PaFd and AvFdI have strikingly different redox potentials; a possible explanation has been sought in the differing hydration models for the two molecules.


==About this Structure==
Structure at pH 6.5 of ferredoxin I from Azotobacter vinelandii at 2.3 A resolution.,Merritt EA, Stout GH, Turley S, Sieker LC, Jehsen LH, Orme-Johnson WH Acta Crystallogr D Biol Crystallogr. 1993 Mar 1;49(Pt 2):272-81. PMID:15299532<ref>PMID:15299532</ref>
1FER is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with F3S and SF4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FER OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure at pH 6.5 of ferredoxin I from Azotobacter vinelandii at 2.3 A resolution., Merritt EA, Stout GH, Turley S, Sieker LC, Jehsen LH, Orme-Johnson WH, Acta Crystallogr D Biol Crystallogr. 1993 Mar 1;49(Pt 2):272-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299532 15299532]
</div>
<div class="pdbe-citations 1fer" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Jensen, L.H.]]
[[Category: Jensen LH]]
[[Category: Merritt, E.A.]]
[[Category: Merritt EA]]
[[Category: Orme-Johnson, W.H.]]
[[Category: Orme-Johnson WH]]
[[Category: Sieker, L.C.]]
[[Category: Sieker LC]]
[[Category: Stout, G.H.]]
[[Category: Stout GH]]
[[Category: Turley, S.]]
[[Category: Turley S]]
[[Category: F3S]]
[[Category: SF4]]
[[Category: electron transfer(iron-sulfur protein)]]
 
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Retrieved from "https://proteopedia.org/1fer"