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[[Image:2gis.gif|left|200px]]
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{{STRUCTURE_2gis|  PDB=2gis  |  SCENE=  }}
'''Structure of the S-adenosylmethionine riboswitch mRNA regulatory element'''


==Structure of the S-adenosylmethionine riboswitch mRNA regulatory element==
<StructureSection load='2gis' size='340' side='right'caption='[[2gis]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2gis]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gis OCA], [https://pdbe.org/2gis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gis RCSB], [https://www.ebi.ac.uk/pdbsum/2gis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gis ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.


==Overview==
Structure of the S-adenosylmethionine riboswitch regulatory mRNA element.,Montange RK, Batey RT Nature. 2006 Jun 29;441(7097):1172-5. PMID:16810258<ref>PMID:16810258</ref>
Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA].
</div>
<div class="pdbe-citations 2gis" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16810258 16810258]
*[[Kink-turn motif|Kink-turn motif]]
[[Category: Batey, R T.]]
*[[Riboswitch 3D structures|Riboswitch 3D structures]]
[[Category: Montange, R K.]]
*[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]]
[[Category: Mrna]]
== References ==
[[Category: Riboswitch]]
<references/>
[[Category: Rna-ligand complex]]
__TOC__
[[Category: S-adenosylmethionine]]
</StructureSection>
[[Category: Sam]]
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:09:11 2008''
[[Category: Large Structures]]
[[Category: Batey RT]]
[[Category: Montange RK]]

Latest revision as of 08:07, 13 August 2026

Structure of the S-adenosylmethionine riboswitch mRNA regulatory element

2gis, resolution 2.90Å

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