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[[Image:2zax.jpg|left|200px]]


{{Structure
==Crystal Structure of Ferric Cytochrome P450cam==
|PDB= 2zax |SIZE=350|CAPTION= <scene name='initialview01'>2zax</scene>, resolution 1.60&Aring;
<StructureSection load='2zax' size='340' side='right'caption='[[2zax]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=CAM:CAMPHOR'>CAM</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
<table><tr><td colspan='2'>[[2zax]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZAX FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
|GENE= camC, cyp101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAM:CAMPHOR'>CAM</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zax OCA], [https://pdbe.org/2zax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zax RCSB], [https://www.ebi.ac.uk/pdbsum/2zax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zax ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPXA_PSEPU CPXA_PSEPU] Involved in a camphor oxidation system.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/za/2zax_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zax ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of Ferric Cytochrome P450cam'''
==See Also==
 
*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
 
__TOC__
==About this Structure==
</StructureSection>
2ZAX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAX OCA].
[[Category: Large Structures]]
 
==Reference==
Evaluation of the functional role of the heme-6-propionate side chain in cytochrome P450cam., Harada K, Sakurai K, Ikemura K, Ogura T, Hirota S, Shimada H, Hayashi T, J Am Chem Soc. 2008 Jan 16;130(2):432-3. Epub 2007 Dec 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18088124 18088124]
[[Category: Camphor 5-monooxygenase]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Harada K]]
[[Category: Harada, K.]]
[[Category: Hayashi T]]
[[Category: Hayashi, T.]]
[[Category: Sakurai K]]
[[Category: Sakurai, K.]]
[[Category: Shimada H]]
[[Category: Shimada, H.]]
[[Category: Tsukihara T]]
[[Category: Tsukihara, T.]]
[[Category: CAM]]
[[Category: HEM]]
[[Category: K]]
[[Category: TRS]]
[[Category: camphor-hydroxylase]]
[[Category: heme]]
[[Category: iron]]
[[Category: membrane]]
[[Category: metal-binding]]
[[Category: monooxygenase]]
[[Category: oxidoreductase]]
[[Category: p450cam]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:53:25 2008''

Latest revision as of 08:39, 13 August 2026

Crystal Structure of Ferric Cytochrome P450cam

2zax, resolution 1.60Å

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