3tom

Crystal structure of an engineered cytochrome cb562 that forms 2D, Zn-mediated sheets

spinqualitylabelsall models 3tom, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3tom is a 4 chain structure with sequence from Escherichia coli. The September 2013 RCSB PDB Molecule of the Month feature on Designed Protein Cages by David Goodsell is 10.2210/rcsb_pdb/mom_2013_9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Ligands: HEM, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C562_ECOLX Electron-transport protein of unknown function. Publication Abstract from PubMed Proteins represent the most sophisticated building blocks available to an organism and to the laboratory chemist. Yet, in contrast to nearly all other types of molecular building blocks, the designed self-assembly of proteins has largely been inaccessible because of the chemical and structural heterogeneity of protein surfaces. To circumvent the challenge of programming extensive non-covalent interactions to control protein self-assembly, we have previously exploited the directionality and strength of metal coordination interactions to guide the formation of closed, homoligomeric protein assemblies. Here, we extend this strategy to the generation of periodic protein arrays. We show that a monomeric protein with properly oriented coordination motifs on its surface can arrange, on metal binding, into one-dimensional nanotubes and two- or three-dimensional crystalline arrays with dimensions that collectively span nearly the entire nano- and micrometre scale. The assembly of these arrays is tuned predictably by external stimuli, such as metal concentration and pH. Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays.,Brodin JD, Ambroggio XI, Tang C, Parent KN, Baker TS, Tezcan FA Nat Chem. 2012 Mar 4;4(5):375-82. doi: 10.1038/nchem.1290. PMID:22522257 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cytochrome b5 3D structures

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