|
|
| (2 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
|
| |
|
| ==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 3== | | ==Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form - oxidized state 3== |
| <StructureSection load='4ttt' size='340' side='right' caption='[[4ttt]], [[Resolution|resolution]] 1.72Å' scene=''> | | <StructureSection load='4ttt' size='340' side='right'caption='[[4ttt]], [[Resolution|resolution]] 1.72Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4ttt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_eutropha_h16 Alcaligenes eutropha h16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TTT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TTT FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4ttt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator_H16 Cupriavidus necator H16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TTT FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=F4S:FE4-S3+CLUSTER'>F4S</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NFV:NI-FE+OXIDIZED+ACTIVE+CENTER'>NFV</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4iub|4iub]], [[4iuc|4iuc]], [[4iud|4iud]], [[3rgw|3rgw]]</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hoxG, PHG002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16]), hoxK, PHG001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381666 Alcaligenes eutropha H16])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ttt OCA], [https://pdbe.org/4ttt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ttt RCSB], [https://www.ebi.ac.uk/pdbsum/4ttt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ttt ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogenase_(acceptor) Hydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.99.6 1.12.99.6] </span></td></tr>
| |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ttt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ttt OCA], [http://pdbe.org/4ttt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ttt RCSB], [http://www.ebi.ac.uk/pdbsum/4ttt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ttt ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. [[http://www.uniprot.org/uniprot/MBHS_CUPNH MBHS_CUPNH]] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. | | [https://www.uniprot.org/uniprot/MBHL_CUPNH MBHL_CUPNH] This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Alcaligenes eutropha h16]] | | [[Category: Cupriavidus necator H16]] |
| [[Category: Kalms, J]] | | [[Category: Large Structures]] |
| [[Category: Scheerer, P]] | | [[Category: Kalms J]] |
| [[Category: Schmidt, A]] | | [[Category: Scheerer P]] |
| [[Category: Aerobic hydrogen bacteria]] | | [[Category: Schmidt A]] |
| [[Category: Bimetallic]]
| |
| [[Category: Dehydrogenase]]
| |
| [[Category: Hydrogen catalysis]]
| |
| [[Category: Hydrogenase]]
| |
| [[Category: Knallgasbacteria]]
| |
| [[Category: Membrane]]
| |
| [[Category: Membrane-bound]]
| |
| [[Category: Metalloenzyme]]
| |
| [[Category: Metalloprotein catalytic center]]
| |
| [[Category: Ni-fe active site]]
| |
| [[Category: Nife]]
| |
| [[Category: Oxidized state]]
| |
| [[Category: Oxidoreductase]]
| |
| [[Category: Oxidoreductase-oxidoreductase complex]]
| |
| [[Category: Oxygen-tolerant hydrogenase]]
| |
| [[Category: Proteobacteria]]
| |
| [[Category: T-cluster]]
| |
