7jz2: Difference between revisions

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New page: '''Unreleased structure''' The entry 7jz2 is ON HOLD Authors: Lyu, M., Su, C.-C., Morgan, C.E., Bolla, J.R., Robinson, C.V., Yu, E.W. Description: Succinate: quinone oxidoreductase SQR...
 
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'''Unreleased structure'''


The entry 7jz2 is ON HOLD
==Succinate: quinone oxidoreductase SQR from E.coli K12==
<StructureSection load='7jz2' size='340' side='right'caption='[[7jz2]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7jz2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JZ2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UQ2:UBIQUINONE-2'>UQ2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jz2 OCA], [https://pdbe.org/7jz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jz2 RCSB], [https://www.ebi.ac.uk/pdbsum/7jz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jz2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SDHA_ECOLI SDHA_ECOLI] Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.<ref>PMID:24374335</ref> <ref>PMID:12560550</ref> <ref>PMID:16407191</ref> <ref>PMID:19710024</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we develop a bottom-up iterative method, Build and Retrieve (BaR), that enables the identification and determination of cryo-EM structures of a variety of inner and outer membrane proteins, including membrane protein complexes of different sizes and dimensions, from a heterogeneous, impure protein sample. We also use the BaR methodology to elucidate structural information from Escherichia coli K12 crude membrane and raw lysate. The findings demonstrate that it is possible to solve high-resolution structures of a number of relatively small (&lt;100 kDa) and less abundant (&lt;10%) unidentified membrane proteins within a single, heterogeneous sample. Importantly, these results highlight the potential of cryo-EM for systems structural proteomics.


Authors: Lyu, M., Su, C.-C., Morgan, C.E., Bolla, J.R., Robinson, C.V., Yu, E.W.
A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.,Su CC, Lyu M, Morgan CE, Bolla JR, Robinson CV, Yu EW Nat Methods. 2021 Jan;18(1):69-75. doi: 10.1038/s41592-020-01021-2. Epub 2021 Jan, 6. PMID:33408407<ref>PMID:33408407</ref>


Description: Succinate: quinone oxidoreductase SQR from E.coli K12
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Bolla, J.R]]
<div class="pdbe-citations 7jz2" style="background-color:#fffaf0;"></div>
[[Category: Yu, E.W]]
 
[[Category: Robinson, C.V]]
==See Also==
[[Category: Morgan, C.E]]
*[[Succinate dehydrogenase 3D structures|Succinate dehydrogenase 3D structures]]
[[Category: Su, C.-C]]
== References ==
[[Category: Lyu, M]]
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Bolla JR]]
[[Category: Lyu M]]
[[Category: Morgan CE]]
[[Category: Robinson CV]]
[[Category: Su C-C]]
[[Category: Yu EW]]
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