8yee

Cryo-EM structure of in vitro reconstituted Light-harvesting complex II trimer

spinqualitylabelsall models 8yee, resolution 2.37Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8yee is a 3 chain structure with sequence from Spinacia oleracea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.37Å Ligands: A1LXP, CLA, LHG, NEX Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Light-harvesting complex II (LHCII) captures sunlight and dissipates excess energy to drive photosynthesis. To elucidate this mechanism, the individual optical properties of pigments in the LHCII protein must be identified. In vitro reconstitution with apoproteins synthesized by Escherichia coli and pigment-lipid mixtures from natural sources is an effective approach; however, the local environment surrounding each pigment within reconstituted LHCII (rLHCII) has only been indirectly estimated using spectroscopic and biochemical methods. Here, we used cryo-electron microscopy to determine the 3D structure of the rLHCII trimer and found that rLHCII exhibited a structure that was virtually identical to that of native LHCII, with a few exceptions: some C-terminal amino acids were not visible, likely due to aggregation of the His-tags; a carotenoid at the V1 site was not visible; and at site 614 showed mixed occupancy by both chlorophyll a and b molecules. Our observations confirmed the applicability of the in vitro reconstitution technique. Structure-based validation of recombinant light-harvesting complex II.,Seki S, Miyata T, Norioka N, Tanaka H, Kurisu G, Namba K, Fujii R PNAS Nexus. 2024 Sep 25;3(9):pgae405. doi: 10.1093/pnasnexus/pgae405. eCollection , 2024 Sep. PMID:39346626 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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