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[[Image:2bmm.gif|left|200px]]
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{{STRUCTURE_2bmm|  PDB=2bmm  |  SCENE=  }}
'''X-RAY STRUCTURE OF A NOVEL THERMOSTABLE HEMOGLOBIN FROM THE ACTINOBACTERIUM THERMOBIFIDA FUSCA'''


==X-ray structure of a novel thermostable hemoglobin from the actinobacterium Thermobifida fusca==
<StructureSection load='2bmm' size='340' side='right'caption='[[2bmm]], [[Resolution|resolution]] 2.48&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bmm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BMM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BMM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.48&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bmm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bmm OCA], [https://pdbe.org/2bmm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bmm RCSB], [https://www.ebi.ac.uk/pdbsum/2bmm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bmm ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/2bmm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bmm ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The gene coding for a hemoglobin-like protein (Tf-trHb) has been identified in the thermophilic actinobacterium Thermobifida fusca and cloned in Escherichia coli for overexpression. The crystal structure of the ferric, acetate-bound derivative, was obtained at 2.48 A resolution. The three-dimensional structure of Tf-trHb is similar to structures reported for the truncated hemoglobins from Mycobacterium tuberculosis and Bacillus subtilis in its central domain. The complete lack of diffraction patterns relative to the N- and C-terminal segments indicates that these are unstructured polypeptides chains, consistent with their facile cleavage in solution. The absence of internal cavities and the presence of two water molecules between the bound acetate ion and the protein surface suggest that the mode of ligand entry is similar to that of typical hemoglobins. The protein is characterized by higher thermostability than the similar mesophilic truncated hemoglobin from B. subtilis, as demonstrated by far-UV CD melting experiments on the cyano-met derivatives. The ligand-binding properties of Tf-trHb, analyzed in stopped flow experiments, demonstrate that Tf-trHb is capable of efficient O2 binding and release between 55 and 60 degrees C, the optimal growth temperature for Thermobifida fusca.


==Overview==
A novel thermostable hemoglobin from the actinobacterium Thermobifida fusca.,Bonamore A, Ilari A, Giangiacomo L, Bellelli A, Morea V, Boffi A FEBS J. 2005 Aug;272(16):4189-201. PMID:16098200<ref>PMID:16098200</ref>
The gene coding for a hemoglobin-like protein (Tf-trHb) has been identified in the thermophilic actinobacterium Thermobifida fusca and cloned in Escherichia coli for overexpression. The crystal structure of the ferric, acetate-bound derivative, was obtained at 2.48 A resolution. The three-dimensional structure of Tf-trHb is similar to structures reported for the truncated hemoglobins from Mycobacterium tuberculosis and Bacillus subtilis in its central domain. The complete lack of diffraction patterns relative to the N- and C-terminal segments indicates that these are unstructured polypeptides chains, consistent with their facile cleavage in solution. The absence of internal cavities and the presence of two water molecules between the bound acetate ion and the protein surface suggest that the mode of ligand entry is similar to that of typical hemoglobins. The protein is characterized by higher thermostability than the similar mesophilic truncated hemoglobin from B. subtilis, as demonstrated by far-UV CD melting experiments on the cyano-met derivatives. The ligand-binding properties of Tf-trHb, analyzed in stopped flow experiments, demonstrate that Tf-trHb is capable of efficient O2 binding and release between 55 and 60 degrees C, the optimal growth temperature for Thermobifida fusca.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2BMM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BMM OCA].
</div>
<div class="pdbe-citations 2bmm" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
A novel thermostable hemoglobin from the actinobacterium Thermobifida fusca., Bonamore A, Ilari A, Giangiacomo L, Bellelli A, Morea V, Boffi A, FEBS J. 2005 Aug;272(16):4189-201. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16098200 16098200]
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
[[Category: Single protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermobifida fusca]]
[[Category: Thermobifida fusca]]
[[Category: Boffi, A.]]
[[Category: Boffi A]]
[[Category: Bonamore, A.]]
[[Category: Bonamore A]]
[[Category: Franceschini, S.]]
[[Category: Franceschini S]]
[[Category: Ilari, A.]]
[[Category: Ilari A]]
[[Category: Bacterial hemoglobin]]
[[Category: Oxygen storage/transport]]
[[Category: Thermostable protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:30:10 2008''
Retrieved from "https://proteopedia.org/2bmm"