4ur1: Difference between revisions
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==Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with dibromoethene== | |||
<StructureSection load='4ur1' size='340' side='right'caption='[[4ur1]], [[Resolution|resolution]] 1.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ur1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurospirillum_multivorans Sulfurospirillum multivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UR1 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.649Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CB0:CIS-DIBROMOETHENE'>CB0</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ur1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ur1 OCA], [https://pdbe.org/4ur1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ur1 RCSB], [https://www.ebi.ac.uk/pdbsum/4ur1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ur1 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region. | |||
Structural basis for organohalide respiration.,Bommer M, Kunze C, Fesseler J, Schubert T, Diekert G, Dobbek H Science. 2014 Oct 2. pii: 1258118. PMID:25278505<ref>PMID:25278505</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4ur1" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sulfurospirillum multivorans]] | |||
[[Category: Bommer M]] | |||
[[Category: Diekert G]] | |||
[[Category: Dobbek H]] | |||
[[Category: Fesseler J]] | |||
[[Category: Kunze C]] | |||
[[Category: Schubert T]] | |||
Latest revision as of 18:16, 8 September 2026
Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with dibromoethene
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