11bq

Escherichia coli 70S ribosome containing an evolved 16S rRNA (EC-S3.5)

spinqualitylabelsall models 11bq, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 11bq is a 10 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3Å Ligands: 1MG, 2MA, 2MG, 3TD, 4D4, 4OC, 5MC, 5MU, 6MZ, G7M, H2U, IAS, MA6, MEQ, MG, MS6, OMC, OMG, OMU, PSU, SPD, SPM, UR3, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The ribosomal RNA sequence governs translation dynamics, yet understanding how changes beyond the conserved catalytic centers influence kinetics and protein yield remains limited. Using orthogonal ribosome phage-assisted continuous evolution (oRibo-PACE), we recently reported chimeric ribosomes derived from Escherichia coli, Pseudomonas aeruginosa, and Vibrio cholerae endowed with elevated translation rates as compared to their starting counterparts. Here, we structurally characterize these kinetically enhanced ribosomes using cryo-electron microscopy and uncover a potential relationship between 16S rRNA stability and translation efficiency. Compared to their naive starting points, evolved ribosomes exhibit extensive RNA structural adaptation, often introduced by mismatches at key helical junctions, which leads to local RNA-protein rearrangements and destabilizes non-canonical base pairs. Compensatory mutations that restore base-pairing stability and eliminate flexibility reduced translational activity to wild-type levels. Across trajectories, increased translational output correlates with subtle, localized changes in the 16S rRNA sequence that introduce limited structural destabilization at specific elements. Taken together, our work provides new insights into rRNA structural malleability and establishes principles for engineering ribosomes with altered translation properties. Structural adaptations for enhanced translation kinetics in evolved ribosomes.,Raskar T, Costello A, Badran AH, Fraser JS bioRxiv [Preprint]. 2026 Mar 6:2026.03.05.706023. doi: , 10.64898/2026.03.05.706023. PMID:41846938 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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