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New page: left|200px<br /><applet load="1t4i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t4i, resolution 2.5Å" /> '''Crystal Structure of ...
 
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[[Image:1t4i.gif|left|200px]]<br /><applet load="1t4i" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1t4i, resolution 2.5&Aring;" />
'''Crystal Structure of a DNA Decamer Containing a Thymine-dimer'''<br />


==Overview==
==Crystal Structure of a DNA Decamer Containing a Thymine-dimer==
It is well known that exposure to UV induces DNA damage, which is the, first step in mutagenesis and a major cause of skin cancer. Among a, variety of photoproducts, cyclobutane-type pyrimidine photodimers (CPD), are the most abundant primary lesion. Despite its biological importance, the precise relationship between the structure and properties of DNA, containing CPD has remained to be elucidated. Here, we report the free, (unbound) crystal structure of duplex DNA containing a CPD lesion at a, resolution of 2.0 A. Our crystal structure shows that the overall helical, axis bends approximately 30 degrees toward the major groove and unwinds, approximately 9 degrees, in remarkable agreement with some previous, theoretical and experimental studies. There are also significant, differences in local structure compared with standard B-DNA, including, pinching of the minor groove at the 3' side of the CPD lesion, a severe, change of the base pair parameter in the 5' side, and serious widening of, both minor and major groves both 3' and 5' of the CPD. Overall, the, structure of the damaged DNA differs from undamaged DNA to an extent that, DNA repair proteins may recognize this conformation, and the various, components of the replicational and transcriptional machinery may be, interfered with due to the perturbed local and global structure.
<StructureSection load='1t4i' size='340' side='right'caption='[[1t4i]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1t4i]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T4I FirstGlance]. <br>
1T4I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T4I OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t4i OCA], [https://pdbe.org/1t4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t4i RCSB], [https://www.ebi.ac.uk/pdbsum/1t4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t4i ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of a DNA decamer containing a cis-syn thymine dimer., Park H, Zhang K, Ren Y, Nadji S, Sinha N, Taylor JS, Kang C, Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15965-70. Epub 2002 Nov 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12456887 12456887]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Kang, C.]]
[[Category: Large Structures]]
[[Category: Nadji, S.]]
[[Category: Kang C]]
[[Category: Park, H.]]
[[Category: Nadji S]]
[[Category: Ren, Y.]]
[[Category: Park H]]
[[Category: Sinha, N.]]
[[Category: Ren Y]]
[[Category: Taylor, J.S.]]
[[Category: Sinha N]]
[[Category: Zhang, K.]]
[[Category: Taylor JS]]
[[Category: dna decamer]]
[[Category: Zhang K]]
[[Category: thymine dimer]]
[[Category: uv-damaged dna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:37:53 2007''

Latest revision as of 08:35, 14 February 2024

Crystal Structure of a DNA Decamer Containing a Thymine-dimer

1t4i, resolution 2.50Å

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