1t5d: Difference between revisions

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New page: left|200px<br /><applet load="1t5d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t5d, resolution 2.206Å" /> '''4-Chlorobenzoyl-CoA...
 
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[[Image:1t5d.gif|left|200px]]<br /><applet load="1t5d" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1t5d, resolution 2.206&Aring;" />
'''4-Chlorobenzoyl-CoA Ligase/Synthetase bound to 4-chlorobenzoate'''<br />


==Overview==
==4-Chlorobenzoyl-CoA Ligase/Synthetase bound to 4-chlorobenzoate==
4-Chlorobenzoate:CoA ligase (CBAL) is a member of a family of, adenylate-forming enzymes that catalyze two-step adenylation and, thioester-forming reactions. In previous studies, we have provided, structural evidence that members of this enzyme family (exemplified by, acetyl-CoA synthetase) use a large domain rotation to catalyze the, respective partial reactions [A. M. Gulick, V. J. Starai, A. R. Horswill, K. M. Homick, and J. C. Escalante-Semerena, (2003) Biochemistry 42, 2866-2873]. CBAL catalyzes the synthesis of 4-chlorobenzoyl-CoA, the first, step in the 4-chlorobenzoate degredation pathway in PCB-degrading, bacteria. We have solved the 2.0 A crystal structure of the CBAL enzyme, from Alcaligenes sp. AL3007 using multiwavelength anomalous dispersion., The results demonstrate that in the absence of any ligands, or bound to, the aryl substrate 4-chlorobenzoate, the enzyme adopts the conformation, poised for catalysis of the adenylate-forming half-reaction. We, hypothesize that coenzyme A binding is required for stabilization of the, alternate conformation, which catalyzes the 4-CBA-CoA thioester-forming, reaction. We have also determined the structure of the enzyme bound to the, aryl substrate 4-chlorobenzoate. The aryl binding pocket is composed of, Phe184, His207, Val208, Val209, Phe249, Ala280, Ile303, Gly305, Met310, and Asn311. The structure of the 4-chlorobenzoate binding site is, discussed in the context of the binding sites of other family members to, gain insight into substrate specificity and evolution of new function.
<StructureSection load='1t5d' size='340' side='right'caption='[[1t5d]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1t5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_sp._AL3007 Alcaligenes sp. AL3007]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T5D FirstGlance]. <br>
1T5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_sp._al3007 Alcaligenes sp. al3007] with CA and 174 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/4-chlorobenzoate--CoA_ligase 4-chlorobenzoate--CoA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.33 6.2.1.33] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T5D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.206&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=174:4-CHLORO-BENZOIC+ACID'>174</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5d OCA], [https://pdbe.org/1t5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t5d RCSB], [https://www.ebi.ac.uk/pdbsum/1t5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t5d ProSAT]</span></td></tr>
Crystal structure of 4-chlorobenzoate:CoA ligase/synthetase in the unliganded and aryl substrate-bound states., Gulick AM, Lu X, Dunaway-Mariano D, Biochemistry. 2004 Jul 13;43(27):8670-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15236575 15236575]
</table>
[[Category: 4-chlorobenzoate--CoA ligase]]
== Function ==
[[Category: Alcaligenes sp. al3007]]
[https://www.uniprot.org/uniprot/Q8GN86_9BURK Q8GN86_9BURK]
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Dunaway-Mariano, D.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Gulick, A.M.]]
Check<jmol>
[[Category: Lu, X.]]
  <jmolCheckbox>
[[Category: 174]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t5/1t5d_consurf.spt"</scriptWhenChecked>
[[Category: CA]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: adenylate-forming; coenzyme a; ligase; domain alternation; conformational change]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:40:35 2007''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t5d ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Alcaligenes sp. AL3007]]
[[Category: Large Structures]]
[[Category: Dunaway-Mariano D]]
[[Category: Gulick AM]]
[[Category: Lu X]]
Retrieved from "https://proteopedia.org/1t5d"