1oyi: Difference between revisions
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New page: left|200px<br /><applet load="1oyi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oyi" /> '''Solution structure of the Z-DNA binding doma... |
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== | ==Solution structure of the Z-DNA binding domain of the vaccinia virus gene E3L== | ||
The N-terminal domain of the vaccinia virus protein E3L (Z alpha(E3L)) is | <StructureSection load='1oyi' size='340' side='right'caption='[[1oyi]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1oyi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OYI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oyi OCA], [https://pdbe.org/1oyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oyi RCSB], [https://www.ebi.ac.uk/pdbsum/1oyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oyi ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q86638_9POXV Q86638_9POXV] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oy/1oyi_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oyi ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The N-terminal domain of the vaccinia virus protein E3L (Z alpha(E3L)) is essential for full viral pathogenicity in mice. It has sequence similarity to the high-affinity human Z-DNA-binding domains Z alpha(ADAR1) and Z alpha(DLM1). Here, we report the solution structure of Z alpha(E3L) and the chemical shift map of its interaction surface with Z-DNA. The global structure and the Z-DNA interaction surface of Z alpha(E3L) are very similar to the high-affinity Z-DNA-binding domains Z alpha(ADAR1) and Z alpha(DLM1). However, the key Z-DNA contacting residue Y48 of Z alpha(E3L) adopts a different side chain conformation in unbound Z alpha(E3L), which requires rearrangement for binding to Z-DNA. This difference suggests a molecular basis for the significantly lower in vitro affinity of Z alpha(E3L) to Z-DNA compared with its homologues. | |||
The solution structure of the N-terminal domain of E3L shows a tyrosine conformation that may explain its reduced affinity to Z-DNA in vitro.,Kahmann JD, Wecking DA, Putter V, Lowenhaupt K, Kim YG, Schmieder P, Oschkinat H, Rich A, Schade M Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2712-7. Epub 2004 Feb 23. PMID:14981270<ref>PMID:14981270</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1oyi" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Vaccinia virus]] | [[Category: Vaccinia virus]] | ||
[[Category: Kahmann | [[Category: Kahmann JD]] | ||
[[Category: Kim | [[Category: Kim Y-G]] | ||
[[Category: Lowenhaupt | [[Category: Lowenhaupt K]] | ||
[[Category: Oschkinat | [[Category: Oschkinat H]] | ||
[[Category: Putter | [[Category: Putter V]] | ||
[[Category: Rich | [[Category: Rich A]] | ||
[[Category: Schade | [[Category: Schade M]] | ||
[[Category: Schmieder | [[Category: Schmieder P]] | ||
[[Category: Wecking | [[Category: Wecking DA]] | ||
