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New page: left|200px<br /><applet load="1ozy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ozy, resolution 2.70Å" /> '''Crystal Structure of...
 
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[[Image:1ozy.jpg|left|200px]]<br /><applet load="1ozy" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ozy, resolution 2.70&Aring;" />
'''Crystal Structure of Phospholipase A2 (MIPLA3) From Micropechis Ikaheka'''<br />


==Overview==
==Crystal Structure of Phospholipase A2 (MIPLA3) From Micropechis Ikaheka==
Comparison of the crystal structures of three Micropechis ikaheka, phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit, different levels of pharmacological effects) shows that their C-terminus, (residues 110-124) is the most variable. M-Type receptor binding affinity, of the isoenzymes has also been investigated and MiPLA4 binds to the, rabbit M-type receptor with high affinity. Examination of surface charges, of the isoenzymes reveals a trend of increase in positive charges with, potency. The isoenzymes are shown to oligomerize in a, concentration-dependent manner in a semi-denaturing gel. The C-termini of, the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster, together, forming a highly exposed area. A BLAST search using the sequence, of the most potent MiPLA2 results in high similarity to Staphylococcus, aureus clotting factor A and cadherin 11. This might explain the, myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.
<StructureSection load='1ozy' size='340' side='right'caption='[[1ozy]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ozy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micropechis_ikaheca Micropechis ikaheca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ozy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozy OCA], [https://pdbe.org/1ozy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ozy RCSB], [https://www.ebi.ac.uk/pdbsum/1ozy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozy ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ozy ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Comparison of the crystal structures of three Micropechis ikaheka phospholipase A2 isoenzymes (MiPLA2, MiPLA3 and MiPLA4, which exhibit different levels of pharmacological effects) shows that their C-terminus (residues 110-124) is the most variable. M-Type receptor binding affinity of the isoenzymes has also been investigated and MiPLA4 binds to the rabbit M-type receptor with high affinity. Examination of surface charges of the isoenzymes reveals a trend of increase in positive charges with potency. The isoenzymes are shown to oligomerize in a concentration-dependent manner in a semi-denaturing gel. The C-termini of the medium (MiPLA4) and highly potent (MiPLA2) isoenzyme molecules cluster together, forming a highly exposed area. A BLAST search using the sequence of the most potent MiPLA2 results in high similarity to Staphylococcus aureus clotting factor A and cadherin 11. This might explain the myotoxicity, anticoagulant and hemoglobinuria effects of MiPLA2s.


==About this Structure==
Structure and function comparison of Micropechis ikaheka snake venom phospholipase A2 isoenzymes.,Lok SM, Gao R, Rouault M, Lambeau G, Gopalakrishnakone P, Swaminathan K FEBS J. 2005 Mar;272(5):1211-20. PMID:15720395<ref>PMID:15720395</ref>
1OZY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Micropechis_ikaheka Micropechis ikaheka] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OZY OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure and function comparison of Micropechis ikaheka snake venom phospholipase A2 isoenzymes., Lok SM, Gao R, Rouault M, Lambeau G, Gopalakrishnakone P, Swaminathan K, FEBS J. 2005 Mar;272(5):1211-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15720395 15720395]
</div>
[[Category: Micropechis ikaheka]]
<div class="pdbe-citations 1ozy" style="background-color:#fffaf0;"></div>
[[Category: Phospholipase A(2)]]
[[Category: Protein complex]]
[[Category: Lok, S.M.]]
[[Category: Swaminathan, K.]]
[[Category: SO4]]
[[Category: micropechis ikaheka]]
[[Category: pancreatic loop]]
[[Category: phospholipase a2]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:44:42 2007''
==See Also==
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Micropechis ikaheca]]
[[Category: Lok SM]]
[[Category: Swaminathan K]]
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