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New page: left|200px<br /><applet load="1kwh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kwh, resolution 2.00Å" /> '''Structure Analysis A...
 
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[[Image:1kwh.jpg|left|200px]]<br /><applet load="1kwh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1kwh, resolution 2.00&Aring;" />
'''Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.'''<br />


==Overview==
==Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.==
Sphingomonas sp. A1 possesses a high molecular mass (average 25,700 Da), alginate uptake system mediated by a novel pit-dependent ABC transporter., The X-ray crystallographic structure of AlgQ2 (57,200 Da), an, alginate-binding protein in the system, was determined by the multiple, isomorphous replacement method and refined at 2.0 A resolution with a, final R-factor of 18.3% for 15 to 2.0 A resolution data. The refined, structure of AlgQ2 was comprised of 492 amino acid residues, 172 water, molecules, and one calcium ion. AlgQ2 was composed of two globular domains, with a deep cleft between them, which is expected to be the, alginate-binding site. The overall structure is basically similar to that, of maltose/maltodextrin-binding protein, except for the presence of an, N2-subdomain. The entire calcium ion-binding site is similar to the site, in the EF-hand motif, but comprises a ten residue loop. This calcium, ion-binding site is about 40 A away from the alginate-binding site.
<StructureSection load='1kwh' size='340' side='right'caption='[[1kwh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1kwh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_sp._A1 Sphingomonas sp. A1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KWH FirstGlance]. <br>
1KWH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KWH OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kwh OCA], [https://pdbe.org/1kwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kwh RCSB], [https://www.ebi.ac.uk/pdbsum/1kwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kwh ProSAT]</span></td></tr>
Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution., Momma K, Mikami B, Mishima Y, Hashimoto W, Murata K, J Mol Biol. 2002 Mar 8;316(5):1051-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11884143 11884143]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Sphingomonas sp.]]
[https://www.uniprot.org/uniprot/Q9KWT5_SPHSX Q9KWT5_SPHSX]
[[Category: Hashimoto, W.]]
== Evolutionary Conservation ==
[[Category: Mikami, B.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Mishima, Y.]]
Check<jmol>
[[Category: Momma, K.]]
  <jmolCheckbox>
[[Category: Murata, K.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kw/1kwh_consurf.spt"</scriptWhenChecked>
[[Category: CA]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: alginate]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: binding protein]]
  </jmolCheckbox>
 
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kwh ConSurf].
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:04:51 2007''
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sphingomonas sp. A1]]
[[Category: Hashimoto W]]
[[Category: Mikami B]]
[[Category: Mishima Y]]
[[Category: Momma K]]
[[Category: Murata K]]

Latest revision as of 13:26, 13 March 2024

Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.

1kwh, resolution 2.00Å

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