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New page: left|200px<br /><applet load="1tee" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tee, resolution 2.90Å" /> '''Crystal structure of...
 
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[[Image:1tee.jpg|left|200px]]<br /><applet load="1tee" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tee, resolution 2.90&Aring;" />
'''Crystal structure of C205F mutant of PKS18 from Mycobacterium tuberculosis'''<br />


==Overview==
==Crystal structure of C205F mutant of PKS18 from Mycobacterium tuberculosis==
The superfamily of plant and bacterial type III polyketide synthases, (PKSs) produces diverse metabolites with distinct biological functions., PKS18, a type III PKS from Mycobacterium tuberculosis, displays an unusual, broad specificity for aliphatic long-chain acyl-coenzyme A (acyl-CoA), starter units (C(6)-C(20)) to produce tri- and tetraketide pyrones. The, crystal structure of PKS18 reveals a 20 A substrate binding tunnel, hitherto unidentified in this superfamily of enzymes. This remarkable, tunnel extends from the active site to the surface of the protein and is, primarily generated by subtle changes of backbone dihedral angles in the, core of the protein. Mutagenic studies combined with structure, determination provide molecular insights into the structural elements that, contribute to the chain length specificity of the enzyme. This first, bacterial type III PKS structure underlines a fascinating example of the, way in which subtle changes in protein architecture can generate, metabolite diversity in nature.
<StructureSection load='1tee' size='340' side='right'caption='[[1tee]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1tee]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TEE FirstGlance]. <br>
1TEE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TEE OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tee OCA], [https://pdbe.org/1tee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tee RCSB], [https://www.ebi.ac.uk/pdbsum/1tee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tee ProSAT]</span></td></tr>
==Reference==
</table>
A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites., Sankaranarayanan R, Saxena P, Marathe UB, Gokhale RS, Shanmugam VM, Rukmini R, Nat Struct Mol Biol. 2004 Sep;11(9):894-900. Epub 2004 Aug 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15286723 15286723]
== Function ==
[[Category: Mycobacterium tuberculosis]]
[https://www.uniprot.org/uniprot/PKS18_MYCTU PKS18_MYCTU] Involved in the biosynthesis of tri- and tetraketide alpha-pyrones. Pks18 catalyzes the extension of medium- and long-chain aliphatic acyl-CoA substrates by using malonyl-CoA as an extender molecule to synthesize polyketide products.<ref>PMID:12941968</ref> <ref>PMID:15286723</ref> <ref>PMID:15984864</ref>
[[Category: Naringenin-chalcone synthase]]
== Evolutionary Conservation ==
[[Category: Single protein]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Rukmini, R.]]
Check<jmol>
[[Category: Sankaranarayanan, R.]]
  <jmolCheckbox>
[[Category: Shanmugam, V.M.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1tee_consurf.spt"</scriptWhenChecked>
[[Category: substrate binding tunnel]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: thiolase fold]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:05:14 2007''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tee ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Rukmini R]]
[[Category: Sankaranarayanan R]]
[[Category: Shanmugam VM]]

Latest revision as of 06:15, 3 April 2024

Crystal structure of C205F mutant of PKS18 from Mycobacterium tuberculosis

1tee, resolution 2.90Å

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