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New page: left|200px<br /><applet load="1xhp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xhp" /> '''Solution Structure of the Extended U6 ISL as...
 
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[[Image:1xhp.gif|left|200px]]<br /><applet load="1xhp" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xhp" />
'''Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae'''<br />


==Overview==
==Solution Structure of the Extended U6 ISL as Observed in the U2/U6 complex from Saccharomyces cerevisiae==
Intron removal in nuclear precursor mRNA is catalyzed through two, transesterification reactions by a multi-megaDalton ribonucleoprotein, machine called the spliceosome. A complex between U2 and U6 small nuclear, RNAs is a core component of the spliceosome. Here we present an NMR, structural analysis of a protein-free U2-U6 complex from Saccharomyces, cerevisiae. The observed folding of the U2-U6 complex is a four-helix, junction, in which the catalytically important AGC triad base-pairs only, within U6 and not with U2. The base-pairing of the AGC triad extends the, U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this, extended U6 ISL reveals structural similarities with domain 5 of group II, self-splicing introns. The observed conformation of the four-helix, junction could be relevant to the first, but not the second, step of, splicing and may help to position the U6 ISL adjacent to the 5' splice, site.
<StructureSection load='1xhp' size='340' side='right'caption='[[1xhp]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xhp]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XHP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xhp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xhp OCA], [https://pdbe.org/1xhp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xhp RCSB], [https://www.ebi.ac.uk/pdbsum/1xhp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xhp ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site.


==About this Structure==
U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction.,Sashital DG, Cornilescu G, McManus CJ, Brow DA, Butcher SE Nat Struct Mol Biol. 2004 Dec;11(12):1237-42. Epub 2004 Nov 14. PMID:15543154<ref>PMID:15543154</ref>
1XHP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XHP OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction., Sashital DG, Cornilescu G, McManus CJ, Brow DA, Butcher SE, Nat Struct Mol Biol. 2004 Dec;11(12):1237-42. Epub 2004 Nov 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15543154 15543154]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1xhp" style="background-color:#fffaf0;"></div>
[[Category: Butcher, S.E.]]
== References ==
[[Category: Cornilescu, G.]]
<references/>
[[Category: Sashital, D.G.]]
__TOC__
[[Category: internal bulge]]
</StructureSection>
[[Category: metal binding site]]
[[Category: Large Structures]]
[[Category: penta-loop]]
[[Category: Butcher SE]]
[[Category: rna stem-loop]]
[[Category: Cornilescu G]]
[[Category: u6 rna]]
[[Category: Sashital DG]]
 
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