3h3a: Difference between revisions

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{{Seed}}
[[Image:3h3a.jpg|left|200px]]


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==The complex structure of CCA-adding enzyme with CTP==
The line below this paragraph, containing "STRUCTURE_3h3a", creates the "Structure Box" on the page.
<StructureSection load='3h3a' size='340' side='right'caption='[[3h3a]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3h3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H3A FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.801&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene></td></tr>
{{STRUCTURE_3h3a|  PDB=3h3a  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h3a OCA], [https://pdbe.org/3h3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h3a RCSB], [https://www.ebi.ac.uk/pdbsum/3h3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h3a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9WZH4_THEMA Q9WZH4_THEMA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h3/3h3a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h3a ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The CCA-adding enzyme synthesizes the CCA sequence at the 3' end of tRNA without a nucleic acid template. The crystal structures of class II Thermotoga maritima CCA-adding enzyme and its complexes with CTP or ATP were determined. The structure-based replacement of both the catalytic heads and nucleobase-interacting neck domains of the phylogenetically closely related Aquifex aeolicus A-adding enzyme by the corresponding domains of the T. maritima CCA-adding enzyme allowed the A-adding enzyme to add CCA in vivo and in vitro. However, the replacement of only the catalytic head domain did not allow the A-adding enzyme to add CCA, and the enzyme exhibited (A, C)-adding activity. We identified the region in the neck domain that prevents (A, C)-adding activity and defines the number of nucleotide incorporations and the specificity for correct CCA addition. We also identified the region in the head domain that defines the terminal A addition after CC addition. The results collectively suggest that, in the class II CCA-adding enzyme, the head and neck domains collaboratively and dynamically define the number of nucleotide additions and the specificity of nucleotide selection.


===The complex structure of CCA-adding enzyme with CTP===
Mechanism for the definition of elongation and termination by the class II CCA-adding enzyme.,Toh Y, Takeshita D, Numata T, Fukai S, Nureki O, Tomita K EMBO J. 2009 Nov 4;28(21):3353-65. Epub 2009 Sep 10. PMID:19745807<ref>PMID:19745807</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_19745807}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3h3a" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 19745807 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_19745807}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3H3A is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H3A OCA].
 
==Reference==
<ref group="xtra">PMID:19745807</ref><references group="xtra"/>
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: TRNA adenylyltransferase]]
[[Category: Toh Y]]
[[Category: Toh, Y.]]
[[Category: Tomita K]]
[[Category: Tomita, K.]]
[[Category: Nucleotide-binding]]
[[Category: Nucleotidyltransferase]]
[[Category: Rna-binding]]
[[Category: Transferase]]
[[Category: Transferase/rna]]
 
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Retrieved from "https://proteopedia.org/3h3a"