1d35: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="1d35" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d35, resolution 1.300Å" /> '''FACILE FORMATION OF...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1d35.gif|left|200px]]<br /><applet load="1d35" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d35, resolution 1.300&Aring;" />
'''FACILE FORMATION OF A CROSSLINKED ADDUCT BETWEEN DNA AND THE DAUNORUBICIN DERIVATIVE MAR70 MEDIATED BY FORMALDEHYDE: MOLECULAR STRUCTURE OF THE MAR70-D(CGTNACG) COVALENT ADDUC'''<br />


==Overview==
==FACILE FORMATION OF A CROSSLINKED ADDUCT BETWEEN DNA AND THE DAUNORUBICIN DERIVATIVE MAR70 MEDIATED BY FORMALDEHYDE: MOLECULAR STRUCTURE OF THE MAR70-D(CGTNACG) COVALENT ADDUC==
MAR70 is a synthetic derivative of the anticancer drug daunorubicin that, contains an additional sugar, attached to the O4' of daunosamine. When, MAR70 was crystallized with the DNA hexamer d(CGTnACG), where nA is, 2-aminoadenine, a covalent methylene bridge was formed between the N3' of, daunosamine and the N2 of 2-aminoadenine. This spontaneous reaction, occurred through the crosslinking action of formaldehyde. The crosslink, was demonstrated by the three-dimensional structure of the 2:1 adduct, between MAR70 and d(CGTnACG) solved at 1.3-A resolution by x-ray, diffraction analysis. The perfect juxtaposition of the two amino groups in, the complex provides a template for efficient addition of formaldehyde., This adduct structure is compared with the analogous structure at 1.5-A, resolution of the complex of MAR70-d(CGTACG), in which no formaldehyde, addition was observed. In both complexes, two MAR70 molecules bind to the, DNA hexamer double helix; the elongated aglycon chromophore is, intercalated between the CpG steps and spans the G.C Watson-Crick base, pairs. The disaccharides occupy nearly the entire minor groove of the, distorted B-DNA hexamer double helix. The second sugar is in contact with, the sugar-phosphate backbone and does not affect the binding interactions, of the daunorubicin portion to DNA. The structure allows us to model the, binding to DNA of drugs having more extensive oligosaccharides. In, addition, it suggests that placing a reactive (e.g., alkylating), functional group at the N3' amino position of daunorubicin might be a, fruitful route for designing anticancer drugs.
<StructureSection load='1d35' size='340' side='right'caption='[[1d35]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1d35]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D35 FirstGlance]. <br>
1D35 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MAR and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D35 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A40:N2-METHYL+2-DEOXYADENOSINE+5-MONOPHOSPHATE'>A40</scene>, <scene name='pdbligand=MAR:4-EPI-4-(2-DEOXYFUCOSE)DAUNOMYCIN'>MAR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d35 OCA], [https://pdbe.org/1d35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d35 RCSB], [https://www.ebi.ac.uk/pdbsum/1d35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d35 ProSAT]</span></td></tr>
Facile formation of a crosslinked adduct between DNA and the daunorubicin derivative MAR70 mediated by formaldehyde: molecular structure of the MAR70-d(CGTnACG) covalent adduct., Gao YG, Liaw YC, Li YK, van der Marel GA, van Boom JH, Wang AH, Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4845-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2052564 2052564]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Boom, J.H.Van.]]
</StructureSection>
[[Category: Gao, Y.G.]]
[[Category: Large Structures]]
[[Category: Li, Y.K.]]
[[Category: Gao Y-G]]
[[Category: Liaw, Y.C.]]
[[Category: Li Y-K]]
[[Category: Marel, G.A.Van.Der.]]
[[Category: Liaw Y-C]]
[[Category: Wang, A.H.J.]]
[[Category: Van Boom JH]]
[[Category: MAR]]
[[Category: Van Der Marel GA]]
[[Category: MG]]
[[Category: Wang AH-J]]
[[Category: complexed with drug]]
[[Category: double helix]]
[[Category: modified]]
[[Category: right handed dna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:22:28 2007''

Latest revision as of 06:48, 7 February 2024

FACILE FORMATION OF A CROSSLINKED ADDUCT BETWEEN DNA AND THE DAUNORUBICIN DERIVATIVE MAR70 MEDIATED BY FORMALDEHYDE: MOLECULAR STRUCTURE OF THE MAR70-D(CGTNACG) COVALENT ADDUC

1d35, resolution 1.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1d35&oldid=4041540"