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New page: left|200px<br /><applet load="1l2d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l2d, resolution 2.0Å" /> '''MutM (Fpg)-DNA Estran...
 
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[[Image:1l2d.gif|left|200px]]<br /><applet load="1l2d" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1l2d, resolution 2.0&Aring;" />
'''MutM (Fpg)-DNA Estranged Guanine Mismatch Recognition Complex'''<br />


==Overview==
==MutM (Fpg)-DNA Estranged Guanine Mismatch Recognition Complex==
MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating, base-excision repair of oxidized guanine residues in DNA. Here we report, five different crystal structures of MutM-DNA complexes that represent, different steps of the repair reaction cascade catalyzed by the protein, and also differ in the identity of the base opposite the lesion (the, 'estranged' base). These structures reveal that the MutM active site, performs the multiple steps of base-excision and 3' and 5' nicking with, minimal rearrangement of the DNA backbone.
<StructureSection load='1l2d' size='340' side='right'caption='[[1l2d]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1l2d]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HPD:1-HYDROXY-PENTANE-3,4-DIOL-5-PHOSPHATE'>HPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2d OCA], [https://pdbe.org/1l2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2d RCSB], [https://www.ebi.ac.uk/pdbsum/1l2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2d ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/1l2d_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l2d ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating base-excision repair of oxidized guanine residues in DNA. Here we report five different crystal structures of MutM-DNA complexes that represent different steps of the repair reaction cascade catalyzed by the protein and also differ in the identity of the base opposite the lesion (the 'estranged' base). These structures reveal that the MutM active site performs the multiple steps of base-excision and 3' and 5' nicking with minimal rearrangement of the DNA backbone.


==About this Structure==
Structural insights into lesion recognition and repair by the bacterial 8-oxoguanine DNA glycosylase MutM.,Fromme JC, Verdine GL Nat Struct Biol. 2002 Jul;9(7):544-52. PMID:12055620<ref>PMID:12055620</ref>
1L2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L2D OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural insights into lesion recognition and repair by the bacterial 8-oxoguanine DNA glycosylase MutM., Fromme JC, Verdine GL, Nat Struct Biol. 2002 Jul;9(7):544-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12055620 12055620]
</div>
<div class="pdbe-citations 1l2d" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Fromme, J.C.]]
[[Category: Fromme JC]]
[[Category: Verdine, G.L.]]
[[Category: Verdine GL]]
[[Category: ZN]]
[[Category: dna glycosylase]]
[[Category: dna repair]]
[[Category: zinc finger]]
 
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