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New page: left|200px<br /><applet load="1tob" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tob" /> '''SACCHARIDE-RNA RECOGNITION IN AN AMINOGLYCOS...
 
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[[Image:1tob.jpg|left|200px]]<br /><applet load="1tob" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tob" />
'''SACCHARIDE-RNA RECOGNITION IN AN AMINOGLYCOSIDE ANTIBIOTIC-RNA APTAMER COMPLEX, NMR, 7 STRUCTURES'''<br />


==Overview==
==SACCHARIDE-RNA RECOGNITION IN AN AMINOGLYCOSIDE ANTIBIOTIC-RNA APTAMER COMPLEX, NMR, 7 STRUCTURES==
BACKGROUND: Aminoglycoside antibiotics are known to target ribosomal, retroviral and catalytic RNAs with high affinity and specificity., Recently, in vitro selection experiments have identified RNA aptamers that, bind to aminoglycoside antibiotics with nanomolar affinity and stringent, specificity, allowing discrimination between closely related family, members. There has, to date, been limited structural information on the, molecular basis of such saccharide-RNA recognition. RESULTS: We describe a, solution-structure determination of the tobramycin-RNA aptamer complex, obtained using NMR and molecular dynamics. The structure gives insight, into the molecular features associated with saccharide-RNA recognition., Tobramycin adopts a defined alignment and binds to the RNA major groove, centered about a stem-loop junction site. A portion of the bound, tobramycin is encapsulated between the floor of the major groove and a, looped-out cytosine residue that forms a flap over the binding site in the, complex. CONCLUSIONS: The emergence of antibiotic-resistant pathogens and, their impact on human health continues to be a major concern in the, medical community. Rational modification of existing antibiotics aimed at, improving their efficacy requires a molecular view of their, receptor-binding sites. We have provided such a molecular view for a, member of the aminoglycoside antibiotic family that targets RNA.
<StructureSection load='1tob' size='340' side='right'caption='[[1tob]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1tob]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TOB FirstGlance]. <br>
1TOB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with TOA, TOC and TOB as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TOB OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TOA:3-DEOXY-3-AMINO+GLUCOSE'>TOA</scene>, <scene name='pdbligand=TOB:1,3-DIAMINO-5,6-DIHYDROXYCYCLOHEXANE'>TOB</scene>, <scene name='pdbligand=TOC:2,3,6-TRIDEOXY-2,6-DIAMINO+GLUCOSE'>TOC</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tob OCA], [https://pdbe.org/1tob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tob RCSB], [https://www.ebi.ac.uk/pdbsum/1tob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tob ProSAT]</span></td></tr>
Saccharide-RNA recognition in an aminoglycoside antibiotic-RNA aptamer complex., Jiang L, Suri AK, Fiala R, Patel DJ, Chem Biol. 1997 Jan;4(1):35-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9070426 9070426]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Fiala, R.]]
</StructureSection>
[[Category: Jiang, L.]]
[[Category: Large Structures]]
[[Category: Patel, D.J.]]
[[Category: Synthetic construct]]
[[Category: Suri, A.K.]]
[[Category: Fiala R]]
[[Category: TOA]]
[[Category: Jiang L]]
[[Category: TOB]]
[[Category: Patel DJ]]
[[Category: TOC]]
[[Category: Suri AK]]
[[Category: ribonucleic acid]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:29:18 2007''

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SACCHARIDE-RNA RECOGNITION IN AN AMINOGLYCOSIDE ANTIBIOTIC-RNA APTAMER COMPLEX, NMR, 7 STRUCTURES

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