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New page: left|200px<br /><applet load="1d77" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d77, resolution 2.400Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1d77.gif|left|200px]]<br /><applet load="1d77" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d77, resolution 2.400&Aring;" />
'''CRYSTAL STRUCTURE OF A B-DNA DODECAMER CONTAINING INOSINE, D(CGCIAATTCGCG), AT 2.4 ANGSTROMS RESOLUTION AND ITS COMPARISON WITH OTHER B-DNA DODECAMERS'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A B-DNA DODECAMER CONTAINING INOSINE, D(CGCIAATTCGCG), AT 2.4 ANGSTROMS RESOLUTION AND ITS COMPARISON WITH OTHER B-DNA DODECAMERS==
The crystal structure of the dodecamer, d(CGCIAATTCGCG), has been, determined at 2.4 A resolution by molecular replacement, and refined to an, R-factor of 0.174. The structure is isomorphous with that of the B-DNA, dodecamer, d(CGCGAATTCGCG), in space group P2(1)2(1)2(1) with cell, dimensions of a = 24.9, b = 40.4, and c = 66.4 A. The initial difference, Fourier maps clearly indicated the presence of inosine instead of guanine., The structure was refined with 44 water molecules, and compared to the, parent dodecamer. Overall the two structures are very similar, and the I:C, forms Watson-Crick base pairs with similar hydrogen bond geometry to the, G:C base pairs. The propeller twist angle is low for I4:C21 and relatively, high for the I16:C9 base pair (-3.2 degrees compared to -23.0 degrees), and the buckle angles alter, probably due to differences in the contacts, with symmetry related molecules in the crystal lattice. The central base, pairs of d(CGCIAATTCGCG) show the large propeller twist angles, and the, narrow minor groove that characterize A-tract DNA, although I:C base pairs, cannot form the major groove bifurcated hydrogen bonds that are possible, for A:T base pairs.
<StructureSection load='1d77' size='340' side='right'caption='[[1d77]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1d77]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D77 FirstGlance]. <br>
1D77 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D77 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d77 OCA], [https://pdbe.org/1d77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d77 RCSB], [https://www.ebi.ac.uk/pdbsum/1d77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d77 ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of a B-DNA dodecamer containing inosine, d(CGCIAATTCGCG), at 2.4 A resolution and its comparison with other B-DNA dodecamers., Xuan JC, Weber IT, Nucleic Acids Res. 1992 Oct 25;20(20):5457-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1437563 1437563]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Weber, I.T.]]
[[Category: Large Structures]]
[[Category: Xuan, J.C.]]
[[Category: Weber IT]]
[[Category: b-dna]]
[[Category: Xuan JC]]
[[Category: double helix]]
[[Category: modified]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:39:00 2007''

Latest revision as of 06:50, 7 February 2024

CRYSTAL STRUCTURE OF A B-DNA DODECAMER CONTAINING INOSINE, D(CGCIAATTCGCG), AT 2.4 ANGSTROMS RESOLUTION AND ITS COMPARISON WITH OTHER B-DNA DODECAMERS

1d77, resolution 2.40Å

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