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New page: left|200px<br /><applet load="1tyj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tyj, resolution 1.60Å" /> '''Crystal Structure An...
 
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[[Image:1tyj.gif|left|200px]]<br /><applet load="1tyj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tyj, resolution 1.60&Aring;" />
'''Crystal Structure Analysis of type II Cohesin A11 from Bacteroides cellulosolvens'''<br />


==Overview==
==Crystal Structure Analysis of type II Cohesin A11 from Bacteroides cellulosolvens==
The incorporation of enzymes into the multi-enzyme cellulosome complex and, its anchoring to the bacterial cell surface are dictated by a set of, binding interactions between two complementary protein modules: the, cohesin and the dockerin. In this work, the X-ray crystal structure of a, type-II cohesin from scaffoldin A of Bacteroides cellulosolvens has been, determined to a resolution of 1.6 angstroms using molecular replacement., The type-II B. cellulosolvens cohesin (Bc-cohesin-II) is the first, detailed description of a crystal structure for a type-II cohesin, and its, features were compared with the known type-I cohesins from Clostridium, thermocellum and Clostridium cellulolyticum (Ct-cohesin-I and, Cc-cohesin-I, respectively). The overall jelly-roll topology of the, type-II Bc-cohesin is very similar to that observed for the type-I, cohesins with three additional secondary structures: an alpha-helix and, two "beta-flaps" that disrupt the normal course of a beta-strand. In, addition, beta-strand 5 is elevated by approximately 4 angstroms on the, surface of the molecule, relative to the type-I Ct and Cc-cohesins. Like, its type-I analogue, the hydrophobic/aromatic core of Bc-cohesin-II, comprises an upper and lower core, but an additional aromatic patch and, conserved tryptophan at the crown of the molecule serves to stabilize the, alpha-helix of the type-II cohesin. Comparison of Bc-cohesin-II with the, known type-I cohesin-dockerin heterodimer suggests that each of the, additional secondary structural elements assumes a flanking position, relative to the putative dockerin-binding surface. The raised ridge formed, by beta-strand 5 confers additional distinctive topographic features to, the proposed binding interface that collectively distinguish between the, type-II and type-I cohesins.
<StructureSection load='1tyj' size='340' side='right'caption='[[1tyj]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tyj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudobacteroides_cellulosolvens Pseudobacteroides cellulosolvens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TYJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tyj OCA], [https://pdbe.org/1tyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tyj RCSB], [https://www.ebi.ac.uk/pdbsum/1tyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tyj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9FDJ9_9FIRM Q9FDJ9_9FIRM]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ty/1tyj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tyj ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The incorporation of enzymes into the multi-enzyme cellulosome complex and its anchoring to the bacterial cell surface are dictated by a set of binding interactions between two complementary protein modules: the cohesin and the dockerin. In this work, the X-ray crystal structure of a type-II cohesin from scaffoldin A of Bacteroides cellulosolvens has been determined to a resolution of 1.6 angstroms using molecular replacement. The type-II B. cellulosolvens cohesin (Bc-cohesin-II) is the first detailed description of a crystal structure for a type-II cohesin, and its features were compared with the known type-I cohesins from Clostridium thermocellum and Clostridium cellulolyticum (Ct-cohesin-I and Cc-cohesin-I, respectively). The overall jelly-roll topology of the type-II Bc-cohesin is very similar to that observed for the type-I cohesins with three additional secondary structures: an alpha-helix and two "beta-flaps" that disrupt the normal course of a beta-strand. In addition, beta-strand 5 is elevated by approximately 4 angstroms on the surface of the molecule, relative to the type-I Ct and Cc-cohesins. Like its type-I analogue, the hydrophobic/aromatic core of Bc-cohesin-II comprises an upper and lower core, but an additional aromatic patch and conserved tryptophan at the crown of the molecule serves to stabilize the alpha-helix of the type-II cohesin. Comparison of Bc-cohesin-II with the known type-I cohesin-dockerin heterodimer suggests that each of the additional secondary structural elements assumes a flanking position relative to the putative dockerin-binding surface. The raised ridge formed by beta-strand 5 confers additional distinctive topographic features to the proposed binding interface that collectively distinguish between the type-II and type-I cohesins.


==About this Structure==
Crystal structure of a type-II cohesin module from the Bacteroides cellulosolvens cellulosome reveals novel and distinctive secondary structural elements.,Noach I, Frolow F, Jakoby H, Rosenheck S, Shimon LW, Lamed R, Bayer EA J Mol Biol. 2005 Apr 22;348(1):1-12. PMID:15808849<ref>PMID:15808849</ref>
1TYJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteroides_cellulosolvens Bacteroides cellulosolvens] with EDO and MOH as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TYJ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of a type-II cohesin module from the Bacteroides cellulosolvens cellulosome reveals novel and distinctive secondary structural elements., Noach I, Frolow F, Jakoby H, Rosenheck S, Shimon LW, Lamed R, Bayer EA, J Mol Biol. 2005 Apr 22;348(1):1-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15808849 15808849]
</div>
[[Category: Bacteroides cellulosolvens]]
<div class="pdbe-citations 1tyj" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Bayer, E.A.]]
<references/>
[[Category: Frolow, F.]]
__TOC__
[[Category: Jakoby, H.]]
</StructureSection>
[[Category: Lamed, R.]]
[[Category: Large Structures]]
[[Category: Noach, I.]]
[[Category: Pseudobacteroides cellulosolvens]]
[[Category: Rosenheck, S.]]
[[Category: Bayer EA]]
[[Category: Shimon, L.J.W.]]
[[Category: Frolow F]]
[[Category: EDO]]
[[Category: Jakoby H]]
[[Category: MOH]]
[[Category: Lamed R]]
[[Category: alpha helix]]
[[Category: Noach I]]
[[Category: beta sandwich]]
[[Category: Rosenheck S]]
[[Category: dockerin-binding module]]
[[Category: Shimon LJW]]
[[Category: flaps]]
 
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