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New page: left|200px<br /><applet load="1u56" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u56, resolution 1.90Å" /> '''Crystal structure of...
 
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[[Image:1u56.gif|left|200px]]<br /><applet load="1u56" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1u56, resolution 1.90&Aring;" />
'''Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (Water-ligated, ferric form)'''<br />


==Overview==
==Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (Water-ligated, ferric form)==
Soluble guanylate cyclases are nitric oxide-responsive signaling proteins, in which the nitric oxide sensor is a heme-binding domain of unknown, structure that we have termed the heme-NO and oxygen binding (H-NOX), domain. H-NOX domains are also found in bacteria, either as isolated, domains, or are fused through a membrane-spanning region to, methyl-accepting chemotaxis proteins. We have determined the crystal, structure of an oxygen-binding H-NOX domain of one such signaling protein, from the obligate anaerobe Thermoanaerobacter tengcongensis at, 1.77-angstroms resolution, revealing a protein fold unrelated to known, structures. Particularly striking is the structure of the protoporphyrin, IX group, which is distorted from planarity to an extent not seen before, in protein-bound heme groups. Comparison of the structure of the H-NOX, domain in two different crystal forms suggests a mechanism whereby, alteration in the degree of distortion of the heme group is coupled to, changes on the molecular surface of the H-NOX domain and potentially to, changes in intermolecular interactions.
<StructureSection load='1u56' size='340' side='right'caption='[[1u56]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1u56]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U56 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U56 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u56 OCA], [https://pdbe.org/1u56 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u56 RCSB], [https://www.ebi.ac.uk/pdbsum/1u56 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u56 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RBX6_CALS4 Q8RBX6_CALS4]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u56_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u56 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Soluble guanylate cyclases are nitric oxide-responsive signaling proteins in which the nitric oxide sensor is a heme-binding domain of unknown structure that we have termed the heme-NO and oxygen binding (H-NOX) domain. H-NOX domains are also found in bacteria, either as isolated domains, or are fused through a membrane-spanning region to methyl-accepting chemotaxis proteins. We have determined the crystal structure of an oxygen-binding H-NOX domain of one such signaling protein from the obligate anaerobe Thermoanaerobacter tengcongensis at 1.77-angstroms resolution, revealing a protein fold unrelated to known structures. Particularly striking is the structure of the protoporphyrin IX group, which is distorted from planarity to an extent not seen before in protein-bound heme groups. Comparison of the structure of the H-NOX domain in two different crystal forms suggests a mechanism whereby alteration in the degree of distortion of the heme group is coupled to changes on the molecular surface of the H-NOX domain and potentially to changes in intermolecular interactions.


==About this Structure==
Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases.,Pellicena P, Karow DS, Boon EM, Marletta MA, Kuriyan J Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12854-9. Epub 2004 Aug 23. PMID:15326296<ref>PMID:15326296</ref>
1U56 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis] with CL and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U56 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of an oxygen-binding heme domain related to soluble guanylate cyclases., Pellicena P, Karow DS, Boon EM, Marletta MA, Kuriyan J, Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12854-9. Epub 2004 Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15326296 15326296]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 1u56" style="background-color:#fffaf0;"></div>
[[Category: Thermoanaerobacter tengcongensis]]
[[Category: Boon, E.M.]]
[[Category: Karow, D.S.]]
[[Category: Kuriyan, J.]]
[[Category: Marletta, M.A.]]
[[Category: Pellicena, P.]]
[[Category: CL]]
[[Category: HEM]]
[[Category: chemotaxis]]
[[Category: h-nox domain]]
[[Category: heme]]
[[Category: oxygen sensor]]
[[Category: signal transduction]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:20:05 2007''
==See Also==
*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
[[Category: Large Structures]]
[[Category: Boon EM]]
[[Category: Karow DS]]
[[Category: Kuriyan J]]
[[Category: Marletta MA]]
[[Category: Pellicena P]]
Retrieved from "https://proteopedia.org/1u56"