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New page: left|200px<br /><applet load="1u5k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u5k, resolution 2.00Å" /> '''Recombinational repa...
 
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[[Image:1u5k.jpg|left|200px]]<br /><applet load="1u5k" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1u5k, resolution 2.00&Aring;" />
'''Recombinational repair protein RecO'''<br />


==Overview==
==Recombinational repair protein RecO==
Recovery of arrested replication requires coordinated action of DNA, repair, replication, and recombination machineries. Bacterial RecO protein, is a member of RecF recombination repair pathway important for replication, recovery. RecO possesses two distinct activities in vitro, closely, resembling those of eukaryotic protein Rad52: DNA annealing and, RecA-mediated DNA recombination. Here we present the crystal structure of, the RecO protein from the extremely radiation resistant bacteria, Deinococcus radiodurans (DrRecO) and characterize its DNA binding and, strand annealing properties. The RecO structure is totally different from, the Rad52 structure. DrRecO is comprised of three structural domains: an, N-terminal domain which adopts an OB-fold, a novel alpha-helical domain, and an unusual zinc-binding domain. Sequence alignments suggest that the, multidomain architecture is conserved between RecO proteins from other, bacterial species and is suitable to elucidate sites of protein-protein, and DNA-protein interactions necessary for RecO functions during the, replication recovery and DNA repair.
<StructureSection load='1u5k' size='340' side='right'caption='[[1u5k]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1u5k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U5K FirstGlance]. <br>
1U5K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans_r1 Deinococcus radiodurans r1] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U5K OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5k OCA], [https://pdbe.org/1u5k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u5k RCSB], [https://www.ebi.ac.uk/pdbsum/1u5k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u5k ProSAT]</span></td></tr>
A novel structure of DNA repair protein RecO from Deinococcus radiodurans., Makharashvili N, Koroleva O, Bera S, Grandgenett DP, Korolev S, Structure. 2004 Oct;12(10):1881-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15458636 15458636]
</table>
[[Category: Deinococcus radiodurans r1]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q9RW50_DEIRA Q9RW50_DEIRA] Involved in DNA repair and RecF pathway recombination (By similarity).[HAMAP-Rule:MF_00201][SAAS:SAAS022572_004_002906]
[[Category: Bera, S.]]
== Evolutionary Conservation ==
[[Category: Grandgenett, D.P.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Korolev, S.]]
Check<jmol>
[[Category: Koroleva, O.]]
  <jmolCheckbox>
[[Category: Makharashvili, N.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u5k_consurf.spt"</scriptWhenChecked>
[[Category: ZN]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: obd-fold]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: zn-binding]]
  </jmolCheckbox>
 
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u5k ConSurf].
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:21:02 2007''
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Deinococcus radiodurans R1]]
[[Category: Large Structures]]
[[Category: Bera S]]
[[Category: Grandgenett DP]]
[[Category: Korolev S]]
[[Category: Koroleva O]]
[[Category: Makharashvili N]]
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