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New page: left|200px<br /><applet load="1i0t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i0t, resolution 0.60Å" /> '''0.6 A STRUCTURE OF Z...
 
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[[Image:1i0t.gif|left|200px]]<br /><applet load="1i0t" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1i0t, resolution 0.60&Aring;" />
'''0.6 A STRUCTURE OF Z-DNA CGCGCG'''<br />


==Overview==
==0.6 A STRUCTURE OF Z-DNA CGCGCG==
The observation of light metal ions in nucleic acids crystals is generally, a fortuitous event. Sodium ions in particular are notoriously difficult to, detect because their X-ray scattering contributions are virtually, identical to those of water and Na(+.)O distances are only slightly, shorter than strong hydrogen bonds between well-ordered water molecules., We demonstrate here that replacement of Na(+) by K(+), Rb(+) or Cs(+) and, precise measurements of anomalous differences in intensities provide a, particularly sensitive method for detecting alkali metal ion-binding sites, in nucleic acid crystals. Not only can alkali metal ions be readily, located in such structures, but the presence of Rb(+) or Cs(+) also allows, structure determination by the single wavelength anomalous diffraction, technique. Besides allowing identification of high occupancy binding, sites, the combination of high resolution and anomalous diffraction data, established here can also pinpoint binding sites that feature only partial, occupancy. Conversely, high resolution of the data alone does not, necessarily allow differentiation between water and partially ordered, metal ions, as demonstrated with the crystal structure of a DNA duplex, determined to a resolution of 0.6 A.
<StructureSection load='1i0t' size='340' side='right'caption='[[1i0t]], [[Resolution|resolution]] 0.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1i0t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I0T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i0t OCA], [https://pdbe.org/1i0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i0t RCSB], [https://www.ebi.ac.uk/pdbsum/1i0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i0t ProSAT]</span></td></tr>
</table>


==About this Structure==
==See Also==
1I0T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I0T OCA].
*[[Z-DNA|Z-DNA]]
 
__TOC__
==Reference==
</StructureSection>
Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments., Tereshko V, Wilds CJ, Minasov G, Prakash TP, Maier MA, Howard A, Wawrzak Z, Manoharan M, Egli M, Nucleic Acids Res. 2001 Mar 1;29(5):1208-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11222771 11222771]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Egli M]]
[[Category: Egli, M.]]
[[Category: Howard A]]
[[Category: Howard, A.]]
[[Category: Maier MA]]
[[Category: Maier, M.A.]]
[[Category: Manoharan M]]
[[Category: Manoharan, M.]]
[[Category: Minasov G]]
[[Category: Minasov, G.]]
[[Category: Prakash TP]]
[[Category: Prakash, T.P.]]
[[Category: Tereshko V]]
[[Category: Tereshko, V.]]
[[Category: Wawrzak Z]]
[[Category: Wawrzak, Z.]]
[[Category: Wilds CJ]]
[[Category: Wilds, C.J.]]
[[Category: SPM]]
[[Category: z-form double helix]]
 
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