Sandbox129: Difference between revisions
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'''Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System of Staphylococcus aureus''' | '''Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System of Staphylococcus aureus''' | ||
Here we report the crystal structure of Enzyme I (EI) from Staphylococcus aureus which is the first component involved in the Sugar Phosphotransferase System (PTS) reaction cascade. | Here we report the crystal structure of Enzyme I (EI) from Staphylococcus aureus which is the first component involved in the Sugar Phosphotransferase System (PTS) reaction cascade. EI is an enzyme of 572 residues and its tertiary structure is composed of 48% α helix and of 13% β-sheet. The crystal structure was obtained with a resolution of 2,40Ǻ. | ||
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== II. Structure of Enzyme I == | == II. Structure of Enzyme I == | ||
<applet load='2wqd' size='400' frame='true' align='right' caption='Structure of ''S. aureus'' EI' /> | <applet load='2wqd' size='400' frame='true' align='right' caption='Structure of ''S. aureus'' EI' /> | ||
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<scene name='Sandbox129/Saei_interacting_with_calcium2/2'>TextToBeDisplayed</scene> | |||
<scene name='Sandbox129/Saei_interacting_with_calcium2/1'>TextToBeDisplayed</scene> | |||
EI is active only in its homodimeric form of 64kDa. As we said, EI consists of a N- and a C-terminal domain. | EI is active only in its homodimeric form of 64kDa. As we said, EI consists of a N- and a C-terminal domain. | ||
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== III.Staphylococcus aureus vs Escherichia coli == | == III.Staphylococcus aureus vs Escherichia coli == | ||
[[Image:Saureus ok.jpg]] | |||
[[Image:Ecoli ok.jpg]] | |||
The X-ray structure of S. aureus EI (SaEI) has been recently solved but structures of other bacterias have been reported before, for instance E. coli EI structure (EcEI). Although SaEI and EcEI X-ray structures are nearly the same, some differences have been observed. | The X-ray structure of S. aureus EI (SaEI) has been recently solved but structures of other bacterias have been reported before, for instance E. coli EI structure (EcEI). Although SaEI and EcEI X-ray structures are nearly the same, some differences have been observed. | ||
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[[Image:tableau SaEI EcEI.jpg]] | [[Image:tableau SaEI EcEI.jpg]] | ||
These two X-ray structures could actually be two possible conformations of the Enzyme I, corresponding to two steps of the phosphotransfer from PEP to HPr. EcEI structure correspond to the conformation II and SaEI structure correspond to the conformation I. | These two X-ray structures could actually be two possible conformations of the Enzyme I, corresponding to two steps of the phosphotransfer from PEP to HPr. EcEI structure correspond to the conformation II and SaEI structure correspond to the conformation I. | ||
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Knowing the structure of S. aureus EI allows a better comprehension of the PTS mechanism. But progresses could be still done such as the possible phenomena which induce the motions of the different domains. | Knowing the structure of S. aureus EI allows a better comprehension of the PTS mechanism. But progresses could be still done such as the possible phenomena which induce the motions of the different domains. | ||
== Reference == | |||
Oberholzer AE, Schneider P, Siebold C, Baumann U, Erni B. (2009).Crystal structure of enzyme I of the phosphoenolpyruvate:Sugar phosphotransferase system in the dephosphorylated state. J Biol Chem | |||
[http://www.ncbi.nlm.nih.gov/pubmed/19801641?dopt=Abstract PMID: 19801641] | |||
== 3D Structures of EI == | |||
[http://www.pdb.org/pdb/explore/explore.do?structureId=2WQD 2wqd] S. aureus EI phosphorylated structure | |||
[http://www.pdb.org/pdb/explore/explore.do?structureId=2HWG 2hwg] E. coli phosphorylated and Mg2+-oxalate bound structure | |||
== Links == | |||
[http://www.proteopedia.org/wiki/index.php/Enzyme_I_of_the_Phosphoenolpyruvate:Sugar_Phosphotransferase_System Proteopedia page] : Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System (E. coli and S. carnosus) | |||
