Sandbox129: Difference between revisions
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'''Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System of Staphylococcus aureus''' | '''Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System of Staphylococcus aureus''' | ||
Here we report the crystal structure of Enzyme I (EI) from Staphylococcus aureus which is the first component involved in the Sugar Phosphotransferase System (PTS) reaction cascade. | Here we report the crystal structure of Enzyme I (EI) from Staphylococcus aureus which is the first component involved in the Sugar Phosphotransferase System (PTS) reaction cascade. EI is an enzyme of 572 residues and its tertiary structure is composed of 48% α helix and of 13% β-sheet. The crystal structure was obtained with a resolution of 2,40Ǻ. | ||
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== II. Structure of Enzyme I == | == II. Structure of Enzyme I == | ||
<applet load='2wqd' size='400' frame='true' align='right' caption='Structure of ''S. aureus'' EI' /> | <applet load='2wqd' size='400' frame='true' align='right' caption='Structure of ''S. aureus'' EI' /> | ||
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<scene name='Sandbox129/Saei_interacting_with_calcium2/2'>TextToBeDisplayed</scene> | |||
<scene name='Sandbox129/Saei_interacting_with_calcium2/1'>TextToBeDisplayed</scene> | |||
EI is active only in its homodimeric form of 64kDa. As we said, EI consists of a N- and a C-terminal domain. | EI is active only in its homodimeric form of 64kDa. As we said, EI consists of a N- and a C-terminal domain. | ||
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Oberholzer AE, Schneider P, Siebold C, Baumann U, Erni B. (2009).Crystal structure of enzyme I of the phosphoenolpyruvate:Sugar phosphotransferase system in the dephosphorylated state. J Biol Chem | Oberholzer AE, Schneider P, Siebold C, Baumann U, Erni B. (2009).Crystal structure of enzyme I of the phosphoenolpyruvate:Sugar phosphotransferase system in the dephosphorylated state. J Biol Chem | ||
PMID: 19801641 | [http://www.ncbi.nlm.nih.gov/pubmed/19801641?dopt=Abstract PMID: 19801641] | ||
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== Links == | == Links == | ||
[http://www.proteopedia.org/wiki/index.php/Enzyme_I_of_the_Phosphoenolpyruvate:Sugar_Phosphotransferase_System Proteopedia page] : Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System (E. coli and S. carnosus) | |||
