1lzl: Difference between revisions

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New page: left|200px<br /><applet load="1lzl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lzl, resolution 1.30Å" /> '''Bacterial Heroin Est...
 
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[[Image:1lzl.gif|left|200px]]<br /><applet load="1lzl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1lzl, resolution 1.30&Aring;" />
'''Bacterial Heroin Esterase'''<br />


==Overview==
==Bacterial Heroin Esterase==
The crystal structures of an acetyl esterase, HerE, and its complex with, an inhibitor dimethylarsinic acid have been determined at 1.30- and 1.45-A, resolution, respectively. Although the natural substrate for the enzyme is, unknown, HerE hydrolyzes the acetyl groups from heroin to yield morphine, and from phenyl acetate to yield phenol. Recently, the activity of the, enzyme toward heroin has been exploited to develop a heroin biosensor, which affords higher sensitivity than other currently available detection, methods. The crystal structure reveals a single domain with the canonical, alpha/beta hydrolase fold with an acyl binding pocket that snugly, accommodates the acetyl substituent of the substrate and three backbone, amides that form a tripartite oxyanion hole. In addition, a covalent, adduct was observed between the active site serine and dimethylarsinic, acid, which inhibits the enzyme. This crystal structure provides the first, example of an As-containing compound in a serine esterase active site and, the first example of covalent modification of serine by arsenic. Thus, the, HerE complex reveals the structural basis for the broad scope inhibition, of serine hydrolases by As(V)-containing organic compounds.
<StructureSection load='1lzl' size='340' side='right'caption='[[1lzl]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1lzl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LZL FirstGlance]. <br>
1LZL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LZL OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lzl OCA], [https://pdbe.org/1lzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lzl RCSB], [https://www.ebi.ac.uk/pdbsum/1lzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lzl ProSAT]</span></td></tr>
==Reference==
</table>
Observation of an arsenic adduct in an acetyl esterase crystal structure., Zhu X, Larsen NA, Basran A, Bruce NC, Wilson IA, J Biol Chem. 2003 Jan 17;278(3):2008-14. Epub 2002 Nov 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12421810 12421810]
== Function ==
[[Category: Rhodococcus sp.]]
[https://www.uniprot.org/uniprot/O06441_RHOSO O06441_RHOSO]
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Basran, A.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Bruce, N.C.]]
Check<jmol>
[[Category: Larsen, N.A.]]
  <jmolCheckbox>
[[Category: Wilson, I.A.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lz/1lzl_consurf.spt"</scriptWhenChecked>
[[Category: Zhu, X.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: alpha/beta hydrolase]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:34:01 2007''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lzl ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodococcus sp]]
[[Category: Basran A]]
[[Category: Bruce NC]]
[[Category: Larsen NA]]
[[Category: Wilson IA]]
[[Category: Zhu X]]

Latest revision as of 07:37, 14 February 2024

Bacterial Heroin Esterase

1lzl, resolution 1.30Å

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