2kpr: Difference between revisions
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==Monomeric intronic human chl1 gene quadruplex DNA NMR, 17 structures== | |||
<StructureSection load='2kpr' size='340' side='right'caption='[[2kpr]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kpr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KPR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kpr OCA], [https://pdbe.org/2kpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kpr RCSB], [https://www.ebi.ac.uk/pdbsum/2kpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kpr ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We report on the solution structure of an unprecedented intramolecular G-quadruplex formed by the guanosine-rich human chl1 intronic d(G(3)-N-G(4)-N(2)-G(4)-N-G(3)-N) 19-mer sequence in K(+)-containing solution. This G-quadruplex, composed of three stacked G-tetrads containing four syn guanines, represents a new folding topology with two unique conformational features. The first guanosine is positioned within the central G-tetrad, in contrast to all previous structures of unimolecular G-quadruplexes, where the first guanosine is part of an outermost G-tetrad. In addition, a V-shaped loop, spanning three G-tetrad planes, contains no bridging nucleotides. The G-quadruplex scaffold is stabilized by a T*G*A triple stacked over the G-tetrad at one end and an unpaired guanosine stacked over the G-tetrad at the other end. Finally, the chl1 intronic DNA G-quadruplex scaffold contains a guanosine base intercalated between an extended G-G step, a feature observed in common with the catalytic site of group I introns. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to intronic G-quadruplex platforms. | |||
Solution structure of a unique G-quadruplex scaffold adopted by a guanosine-rich human intronic sequence.,Kuryavyi V, Patel DJ Structure. 2010 Jan 13;18(1):73-82. PMID:20152154<ref>PMID:20152154</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2kpr" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Kuryavyi V]] | |||
[[Category: Patel DJ]] | |||
Latest revision as of 12:51, 20 December 2023
Monomeric intronic human chl1 gene quadruplex DNA NMR, 17 structures
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