2wzg: Difference between revisions
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< | ==Legionella glucosyltransferase (Lgt1) crystal structure== | ||
<StructureSection load='2wzg' size='340' side='right'caption='[[2wzg]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2wzg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WZG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
-- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wzg OCA], [https://pdbe.org/2wzg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wzg RCSB], [https://www.ebi.ac.uk/pdbsum/2wzg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wzg ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5WWY0_LEGPL Q5WWY0_LEGPL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Legionnaires' disease is caused by a lethal colonization of alveolar macrophages with the Gram-negative bacterium Legionella pneumophila. LpGT (L. pneumophila glucosyltransferase; also known as Lgt1) has recently been identified as a virulence factor, shutting down protein synthesis in the human cell by specific glucosylation of EF1A (elongation factor 1A), using an unknown mode of substrate recognition and a retaining mechanism for glycosyl transfer. We have determined the crystal structure of LpGT in complex with substrates, revealing a GT-A fold with two unusual protruding domains. Through structure-guided mutagenesis of LpGT, several residues essential for binding of the UDP-glucose-donor and EF1A-acceptor substrates were identified, which also affected L. pneumophila virulence as demonstrated by microinjection studies. Together, these results suggested that a positively charged EF1A loop binds to a negatively charged conserved groove on the LpGT structure, and that two asparagine residues are essential for catalysis. Furthermore, we showed that two further L. pneumophila glycosyltransferases possessed the conserved UDP-glucose-binding sites and EF1A-binding grooves, and are, like LpGT, translocated into the macrophage through the Icm/Dot (intracellular multiplication/defect in organelle trafficking) system. | |||
Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase.,Hurtado-Guerrero R, Zusman T, Pathak S, Ibrahim AF, Shepherd S, Prescott A, Segal G, van Aalten DM Biochem J. 2010 Feb 24;426(3):281-92. PMID:20030628<ref>PMID:20030628</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
== | </div> | ||
<div class="pdbe-citations 2wzg" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Legionella pneumophila]] | [[Category: Legionella pneumophila]] | ||
[[Category: Hurtado-Guerrero R]] | |||
[[Category: Hurtado-Guerrero | [[Category: Ibrahim AFM]] | ||
[[Category: Ibrahim | [[Category: Pathak S]] | ||
[[Category: Pathak | [[Category: Prescott A]] | ||
[[Category: Prescott | [[Category: Segal G]] | ||
[[Category: Segal | [[Category: Shepherd S]] | ||
[[Category: Shepherd | [[Category: Van Aalten DMF]] | ||
[[Category: | [[Category: Zusman T]] | ||
[[Category: | |||
Latest revision as of 10:36, 16 August 2023
Legionella glucosyltransferase (Lgt1) crystal structure
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